@article{fdi:010044256, title = {{T}he rpo{B} gene as a tool for clinical microbiologists}, author = {{A}dekambi, {T}. and {D}rancourt, {M}. and {R}aoult, {D}idier}, editor = {}, language = {{ENG}}, abstract = {{T}he rpo{B} gene, encoding the beta-subunit of {RNA} polymerase, has emerged as a core gene candidate for phylogenetic analyses and identification of bacteria, especially when studying closely related isolates. {T}ogether with the 16{S} r{RNA} gene, rpo{B} has helped to delineate new bacterial species and refine bacterial community analysis, as well as enabling the monitoring of rifampicin resistance-conferring mutations. {S}equencing of rpo{B} enables efficient estimation of bacterial {G}+{C}% content, {DNA}-{DNA} hybridization value and average nucleotide identity (percentage of the total genomic sequence shared between two strains) when taxonomic relationships have been firmly established. {N}ew identification tools targeting a rpo{B} gene fragment located between positions 2300 and 3300 have been developed recently. {T}herefore, inclusion of the rpo{B} gene sequence would be useful when describing new bacterial species.}, keywords = {}, booktitle = {}, journal = {{T}rends in {M}icrobiology}, volume = {17}, numero = {1}, pages = {37--45}, ISSN = {0966-842{X}}, year = {2009}, DOI = {10.1016/j.tim.2008.09.008}, URL = {https://www.documentation.ird.fr/hor/fdi:010044256}, }