@article{fdi:010044207,
  title = {{A} quorum-quenching approach to identify quorum-sensing-regulated functions in {A}zospirillum lipoferum},
  author = {{B}oyer, {M}. and {B}ally, {R}. and {P}errotto, {S}. and {C}haintreuil, {C}lemence and {W}isniewski-{D}ye, {F}.},
  editor = {},
  language = {{ENG}},
  abstract = {{A} quorum-quenching approach was exploited in order to identify functions regulated by quorum-sensing ({QS}) in the plant growth-promoting bacterium {A}zospirillum lipoferum. {T}he {A}tt{M} lactonase from {A}grobacterium tumefaciens was shown to enzymatically inactivate {N}-acyl homoserine lactones ({AHL}s) produced by two {A}. lipoferum strains. {T}he targeted analysis of several phenotypes revealed that in strain 13518, a rice endophyte, {AHL} inactivation abolished pectinase activity, increased siderophore synthesis and reduced indoleacetic acid production (in stationary phase) but no effect was observed on cellulase activity or on swimming and swarming motilities. {N}one of the tested phenotypes appeared to be under {QS} regulation in strain {TVV}3 isolated from the rice rhizosphere. {M}oreover, {AHL} inactivation had no deleterious effect on the phytostimulatory effect of the two strains in vitro. {A} global proteomic approach revealed little modification of protein patterns when comparing att{M}-expressing {TVV}3 and the wild-type strain, but numerous proteins appeared to be regulated by the {AHL}-mediated {QS} system in strain {B}518. {S}everal proteins identified by {MS}-{MS} analysis were revealed to be implicated in transport (such as {O}ma{A}) and chemotaxis ({C}hv{E}). {A}ltogether, the results indicate that in {A}. lipoferum, {QS} regulation is strain-specific and is dedicated to regulating functions linked to rhizosphere competence and adaptation to plant roots.},
  keywords = {{A}zospirillum ; {IAA} ; {P}ectinase ; {PGPR} ; {Q}uorum sensing},
  booktitle = {},
  journal = {{R}esearch in {M}icrobiology},
  volume = {159},
  numero = {9-10},
  pages = {699--708},
  ISSN = {0923-2508},
  year = {2008},
  DOI = {10.1016/j.resmic.2008.08.003},
  URL = {https://www.documentation.ird.fr/hor/fdi:010044207},
}