@article{fdi:010044130,
  title = {{DNA} methylation in different origin clonal offspring from a mature {S}equoiadendron giganteum genotype},
  author = {{M}onteuuis, {O}. and {D}oulbeau, {S}ylvie and {V}erdeil, {J}. {L}.},
  editor = {},
  language = {{ENG}},
  abstract = {{A} meristem-issued rejuvenated line was obtained in 1986 from a 100-year-old {S}equoiadendron giganteum tree and has been since then micropropagated in tissue culture conditions maintaining its juvenile-like characteristics. {B}y contrast, grafts and rooted microcuttings from the same genotype planted in outdoor conditions for several years exhibited mature foliage traits and the grafts started to produce cones, which are obvious indicators of physiological aging. {T}hese three different clonal lines were compared with regard to global {DNA} methylation assessed by {HPLC}. {T}he in vitro rejuvenated line showed a much higher level of {DNA} methylation (23% as average value) than the two other outdoor origins from the same clone which displayed similar degrees of global methylation (average values of 13.4% for the grafts and 13.8% for the cuttings). {O}verall these {DNA} global methylation values obtained for the first time in {S}. giganteum are consistent with the level of methylation reported for many plants using the same {HPLC} protocols. {T}he fact that shoots exhibiting a juvenile-like leaf morphology can be characterized by higher {DNA} methylation than mature-like ones is discussed in relation to physiological aging, referring to other studies on the same topic.},
  keywords = {{A}ging ; {C}oniferous species ; {D}na methylation ; {M}aturational traits ; {P}hase change},
  booktitle = {},
  journal = {{T}rees - {S}tructure and {F}unction},
  volume = {22},
  numero = {6},
  pages = {779--784},
  ISSN = {0931-1890},
  year = {2008},
  DOI = {10.1007/s00468-008-0238-3},
  URL = {https://www.documentation.ird.fr/hor/fdi:010044130},
}