@article{fdi:010042977,
  title = {{E}ctomycorrhizal symbiosis enhanced the efficiency of inoculation with two {B}radyrhizobium strains and {A}cacia holosericea growth},
  author = {{A}ndre, {S}. and {G}aliana, {A}. and {L}e {R}oux, {C}. and {P}rin, {Y}ves and {N}eyra, {M}arc and {D}uponnois, {R}obin},
  editor = {},
  language = {{ENG}},
  abstract = {{T}wo strains of {B}radyrhizobium sp., {A}ust 13{C} and {A}ust 11{C}, were dually or singly inoculated with an ectomycorrhizal fungus, {P}isolithus albus to assess the interactions between ectomycorrhizal symbiosis and the nodulation process in glasshouse conditions. {S}equencing of strains {A}ust 13{C} and {A}ust 11{C} confirmed their previous placement in the genus {B}radyrhizobium. {A}fter 4 months' culture, the ectomycorrhizal symbiosis promoted plant growth and the nodulation process of both {B}radyrhizobium strains, singly or dually inoculated. {PCR}/{RFLP} analysis of the nodules randomly collected in each treatment with {A}ust 13{C} and/or {A}ust 11{C}: (1) showed that all the nodules exhibited the same patterns as those of the {B}radyrhizobium strains, and (2) did not detect contaminant rhizobia. {W}hen both {B}radyrhizobium isolates were inoculated together, but without {P}. albus {IR}100, {A}ust 11{C} was recorded in 13% of the treated nodules compared to 87% for {A}ust 13{C}, whereas {A}ust 11{C} and {A}ust 13{C} were represented in 20 and 80% of the treated nodules, respectively, in the ectomycorrhizal treatment. {T}herefore {A}ust 13{C} had a high competitive ability and a great persistence in soil. {T}he presence of the fungus did not significantly influence the frequencies of each {B}radyrhizobium sp. root nodules. {A}lthough the mechanisms remain unknown, these results showed that the ectomycorrhizal and biological nitrogen-fixing symbioses were very dependent on each other. {F}rom a practical point of view, the role of ectomycorrhizal symbiosis is of great importance to {N}-2 fixation and, consequently, these kinds of symbiosis must be associated in any controlled inoculation.},
  keywords = {{A}cacia ; ectomycorrhizas ; rhizobia ; microbial interaction},
  booktitle = {},
  journal = {{M}ycorrhiza},
  volume = {15},
  numero = {5},
  pages = {357--364},
  ISSN = {0940-6360},
  year = {2005},
  DOI = {10.1007/s00572-004-0340-3},
  URL = {https://www.documentation.ird.fr/hor/fdi:010042977},
}