Villarreal D. auteur aut IRD Nirde P. auteur aut IRD Hide Mallorie auteur aut IRD Barnabé Christian auteur aut IRD Tibayrenc Michel auteur aut IRD text journalArticle eng text/pdf reformatted digital access We analyzed the differential gene expression among representative Trypanosoma cruzi stocks in relation to benznidazole exposures using a random differentially expressed sequences (RADES) technique. Studies were carried out with drug pressure both at the natural susceptibility level of the wild-type parasite (50% inhibitory concentration for the wild type) and at different resistance levels. The pattern of differential gene expression performed with resistant stocks was compared to the population structure of this parasite, established by random amplified polymorphic DNA analysis and multilocus enzyme electrophoresis. A RADES band polymorphism was observed, and over- or underexpression was linked to the resistance level of the stock. The analysis of RADES bands suggested that different products may be involved in benznidazole resistance mechanisms. No significant association was found between phylogenetic clustering and benznidazole susceptibility. Benznidazole resistance may involve several mechanisms, depending on the level of drug exposure. specialized 052 49 7 2701-2709 2005 0066-4804 https://www.documentation.ird.fr/hor/fdi:010042973 10.1128/AAC.49.7.2701-2709.2005 0066-4804 [F B010042973] https://www.documentation.ird.fr/hor/fdi:010042973 https://horizon.documentation.ird.fr/exl-doc/pleins_textes/2024-12/010042973.pdf IRD - Base Horizon / Pleins textes 2006-03-29 2024-12-18 fdi:010042973 fre