@article{fdi:010042969,
  title = {{O}ptimal hydration status for cryopreservation of intermediate oily seeds : citrus as a case study},
  author = {{H}or, {Y}.{L}. and {K}im, {Y}.{J}. and {U}gap, {A}. and {C}habrillange, {N}athalie and {S}inniah, {U}.{R}. and {E}ngelmann, {F}lorent and {D}ussert, {S}t{\'e}phane},
  editor = {},
  language = {{ENG}},
  abstract = {{B}ackground and {A}ims {T}he purpose of this study was to investigate the basis of the optimal hydration status for cryopreservation of intermediate oily seeds using {C}itrus as a model. {M}ethods {T}he relationships between equilibrium relative humidity ({RH}), seed water content, presence of freezable water as determined by {DSC} analysis, and germination percentage after immersion in liquid nitrogen ({LN}) were investigated in {C}itrus aurantifolia, {C}. grandis, {C}. madurensis and {C}. reticulata. {T}he relationship between the lipid content of seeds and their unfrozen water content was also investigated. {K}ey {R}esults {I}ndependent of their level of seed desiccation tolerance, the optimal desiccation {RH} for seed tolerance to {LN} exposure was 75-80 % in the four species studied. {T}his optimal hydration status always coincided with that at which presence of frozen water could not be detected in seed tissues during the cooling/thawing process. {T}he unfrozen water content of seeds was variable between species and negatively correlated to seed lipid content. {U}sing the present data, those obtained previously in seven coffee species and those reported by other authors for five other species, a significant linear relationship was found between the lipid content and the unfrozen water content of seeds. {C}onclusions {T}his study provides additional evidence that intermediate oily seeds do not withstand the presence of freezable water in their tissues during the cooling/warming process. {M}oreover, it offers two important applied perspectives: (1) independent of their level of desiccation tolerance, testing germination of seeds of a given oily seed species after equilibration in 75-80 % {RH} at 25 degrees {C} and {LN} exposure, gives a rapid and reliable evaluation of the possibility of cryopreserving whole seeds of this given species; (2) it is now possible to calculate the interval of water contents in which non-orthodox oily seeds of a given species are likely to withstand {LN} exposure as a function of their lipid content.},
  keywords = {seed ; intermediate ; non orthodox ; {C}itrus ; cryopreservation ; water activity ; relative humidity ; desiccation ; lipid content ; {DSC} ; ice ; unfrozen water},
  booktitle = {},
  journal = {{A}nnals of {B}otany},
  volume = {95},
  numero = {7},
  pages = {1153--1161},
  ISSN = {0305-7364},
  year = {2005},
  DOI = {10.1093/aob/mci126},
  URL = {https://www.documentation.ird.fr/hor/fdi:010042969},
}