@article{fdi:010042963,
  title = {{P}hysical and partial genetic map of {S}podoptera frugiperda nucleopolyhedrovirus ({S}f{MNPV}) genome},
  author = {{S}imon, {O}. and {C}hevenet, {F}ran{\c{c}}ois and {W}illiams, {T}. and {C}aballero, {P}. and {L}opez {F}erber, {M}.},
  editor = {},
  language = {{ENG}},
  abstract = {{N}icaraguan isolate of {S}podoptera frugiperda multicapsid nucleopolyhedrovirus ({S}f{MNPV}) is undergoing field trials for control of this pest in the {A}mericas. {T}his isolate is composed of multiple genotypes, some of which are deletion mutants. {I}dentification of the genetic changes in deleted genotypes cannot be accomplished without the construction of a detailed physical map. {I}n the present study, combinations of restriction endonuclease analysis and {S}outhern blot analysis was performed. {T}his map was refined by sequencing the termini of cloned restriction fragments. {T}he {S}f{MNPV} genome was estimated to be 129.3 kb, 8 kb larger than the previously characterized {S}f-2 variant from the {U}nited {S}tates, due to a deletion between 14.8 and 21.0 m.u. in the physical map described in this study. {A} total of 27.92 kb were sequenced, which represented 21.5% of the whole genome and included 38 {ORF}s. {C}omparison with other sequenced baculoviruses revealed that {S}f{MNPV} displayed the highest sequence identity (66%) and gene arrangement (78%) with {S}podoptera exigua {MNPV}, sharing 36 putative {ORF}s. {I}n addition, the genome organization was similar to that of {S}e{MNPV}, with minor differences. {P}hylogenetic analysis confirmed the close relatedness between {S}e{MNPV} and {S}f{MNPV}, suggesting they evolved from a common ancestor.},
  keywords = {genetic map ; nucleopolyhedrovirus ; physical map ; {S}podoptera frugiperda},
  booktitle = {},
  journal = {{V}irus {G}enes},
  volume = {30},
  numero = {3},
  pages = {403--417},
  ISSN = {0920-8569},
  year = {2005},
  DOI = {10.1007/s11262-004-6784-x},
  URL = {https://www.documentation.ird.fr/hor/fdi:010042963},
}