@article{fdi:010042766,
  title = {{E}ffects of {M}edicago truncatula genetic diversity, rhizobial competition, and strain effectiveness on the diversity of a natural {S}inorhizobium species community},
  author = {{R}angin, {C}{\'e}cile and {B}runel, {B}. and {C}leyet-{M}arel, {J}. {C}. and {P}errineau, {M}. {M}. and {B}{\'e}na, {G}illes},
  editor = {},
  language = {{ENG}},
  abstract = {{W}e investigated the genetic diversity and symbiotic efficiency of 223 {S}inorhizobium sp. isolates sampled from a single {M}editerranean soil and trapped with four {M}edicago truncatula lines. {DNA} molecular polymorphism was estimated by capillary electrophoresis-single-stranded conformation polymorphism and restriction fragment length polymorphism on five loci ({IGS}({NOD}), typ{A}, vir{B}11, avh{B}11, and the 16{S} r{RNA} gene). {M}ore than 90% of the rhizobia isolated belonged to the {S}inorhizobium medicae species (others belonged to {S}inorhizobium meliloti), with different proportions of the two species among the four {M}. truncatula lines. {T}he {S}. meliloti population was more diverse than that of {S}. medicae, and significant genetic differentiation among bacterial populations was detected. {S}ingle inoculations performed in tubes with each bacterial genotype and each plant line showed significant bacterium-plant line interactions for nodulation and {N}-2 fixation levels. {C}ompetition experiments within each species highlighted either strong or weak competition among genotypes within {S}. medicae and {S}. meliloti, respectively. {I}nterspecies competition experiments showed {S}. meliloti to be more competitive than {S}. medicae for nodulation. {A}lthough not highly divergent at a nucleotide level, isolates collected from this single soil sample displayed wide polymorphism for both nodulation and {N}-2 fixation. {E}ach {M}. truncatula line might influence {S}inorhizobium soil population diversity differently via its symbiotic preferences. {O}ur data suggested that the two species did not evolve similarly, with {S}. meliloti showing polymorphism and variable selective pressures and {S}. medicae showing traces of a recent demographic expansion. {S}train effectiveness might have played a role in the species and genotype proportions, but in conjunction with strain adaptation to environmental factors.},
  keywords = {},
  booktitle = {},
  journal = {{A}pplied and {E}nvironmental {M}icrobiology},
  volume = {74},
  numero = {18},
  pages = {5653--5661},
  ISSN = {0099-2240},
  year = {2008},
  DOI = {10.1128/aem.01107-08},
  URL = {https://www.documentation.ird.fr/hor/fdi:010042766},
}