Croce O. auteur aut IRD Chevenet François auteur aut IRD Christen R. auteur aut IRD text journalArticle eng text/pdf born digital access The efficiency of molecular methods involving DNA/DNA hybridizations depends on the accurate prediction of the melting temperature (T-m) of the duplex. Many softwares are available for Tm calculations, but difficulties arise when one wishes to check if a given oligomer (PCR primer or probe) hybridizes well or not on more than a single sequence. Moreover, the presence of mismatches within the duplex is not sufficient to estimate specificity as it does not always significantly decrease the Tm. OHM (OligoHeatMap) is an online tool able to provide estimates of T-m for a set of oligomers and a set of aligned sequences, not only as text files of complete results but also in a graphical way: Tm values are translated into colors and displayed as a heat map image, either stand alone or to be used by softwares such as TreeDyn to be included in a phylogenetic tree. OHM is freely available at http://bioinfo.unice.fr/ohm/, with links to the full source code and online help. specialized 020 36 Suppl S W154-W156 2008 0305-1048 https://www.documentation.ird.fr/hor/fdi:010042722 10.1093/nar/gkn221 0305-1048 [F B010042722] https://www.documentation.ird.fr/hor/fdi:010042722 https://www.documentation.ird.fr/intranet/publi/2008/08/010042722.pdf Accès réservé (Intranet de l'IRD) IRD - Base Horizon / Pleins textes 2008-09-19 2022-06-08 fdi:010042722 fre