@article{fdi:010042722,
  title = {{O}ligo{H}eat{M}ap ({OHM}) : an online tool to estimate and display hybridizations of oligonucleotides onto {DNA} sequences},
  author = {{C}roce, {O}. and {C}hevenet, {F}ran{\c{c}}ois and {C}hristen, {R}.},
  editor = {},
  language = {{ENG}},
  abstract = {{T}he efficiency of molecular methods involving {DNA}/{DNA} hybridizations depends on the accurate prediction of the melting temperature ({T}-m) of the duplex. {M}any softwares are available for {T}m calculations, but difficulties arise when one wishes to check if a given oligomer ({PCR} primer or probe) hybridizes well or not on more than a single sequence. {M}oreover, the presence of mismatches within the duplex is not sufficient to estimate specificity as it does not always significantly decrease the {T}m. {OHM} ({O}ligo{H}eat{M}ap) is an online tool able to provide estimates of {T}-m for a set of oligomers and a set of aligned sequences, not only as text files of complete results but also in a graphical way: {T}m values are translated into colors and displayed as a heat map image, either stand alone or to be used by softwares such as {T}ree{D}yn to be included in a phylogenetic tree. {OHM} is freely available at http://bioinfo.unice.fr/ohm/, with links to the full source code and online help.},
  keywords = {},
  booktitle = {},
  journal = {{N}ucleic {A}cids {R}esearch},
  volume = {36},
  numero = {{S}uppl {S}},
  pages = {{W}154--{W}156},
  ISSN = {0305-1048},
  year = {2008},
  DOI = {10.1093/nar/gkn221},
  URL = {https://www.documentation.ird.fr/hor/fdi:010042722},
}