%0 Journal Article
%9 ACL : Articles dans des revues avec comité de lecture répertoriées par l'AERES
%A Beck, I. A.
%A Crowell, C.
%A Kittoe, R.
%A Bredell, H.
%A Machaba, M.
%A Willamson, C.
%A Janssens, W.
%A Jallow, S.
%A van der Groen, G.
%A Shao, Y.
%A Jacob, M.
%A Samuel, N. M.
%A de Rivera, I. L.
%A Ngo-Giang-Huong, Nicole
%A Cassol, S.
%A Alemnji, G.
%A Frenkel, L. M.
%T Optimization of the oligonucleotide ligation assay, a rapid and inexpensive test for detection of HIV-1 drug resistance mutations, for non-North American variants
%D 2008
%L fdi:010042699
%G ENG
%J JAIDS Journal of Acquired Immune Deficiency Syndromes
%@ 1525-4135
%K Hiv drug resistance ; Hiv-1 non-b subtypes ; Resistance testing ; Oligonucleotide ligation assay ; Minor genotypes ; Point mutation assay ; Dried blood spots
%M ISI:000257808100006
%N 4
%P 418-427
%R 10.1097/QAI.0b013e31817ed7d7
%U https://www.documentation.ird.fr/hor/fdi:010042699
%> https://www.documentation.ird.fr/intranet/publi/2008/08/010042699.pdf
%V 48
%W Horizon (IRD)
%X Objective: We evaluated the feasibility of the oligonucleotide ligation assay (OLA), a specific, sensitive, and economical ligase-based point mutation assay designed to detect HIV-1 drug-resistance mutations at 12 codons of HIV-1 subtype B pol, for potential use in resource-poor settings. Methods: Specimens from HIV-1-infected individuals collected by 7 international laboratories, including subtypes A, B, C, D, F, G, J, and recombinants AE and AG, were tested by the OLA developed for HIV-1 subtype B. Common polymorphisms that interfered with reactivity of the OLA were identified and modified probes designed and evaluated. Results: 92.5% (2410) of 2604 codons in specimens from 217 individuals were successfully genotyped by the subtype B OLA. A high rate (range 8.3%-31.2%) of indeterminate results (negative OLA reaction for both mutant and wild type) was observed for 5 codons. Modified probes at reverse transcriptase codons 151 and 184 and protease codon 90 increased the rate of valid OLA to 96.1%. Conclusions: The OLA designed for HIV-1 subtype B genotyped most pol codons in non-B subtypes from Asia and Africa but was improved by addition of several modified probes. International laboratories experienced in molecular techniques were able to perform the OLA.
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