@article{fdi:010042699,
  title = {{O}ptimization of the oligonucleotide ligation assay, a rapid and inexpensive test for detection of {HIV}-1 drug resistance mutations, for non-{N}orth {A}merican variants},
  author = {{B}eck, {I}. {A}. and {C}rowell, {C}. and {K}ittoe, {R}. and {B}redell, {H}. and {M}achaba, {M}. and {W}illamson, {C}. and {J}anssens, {W}. and {J}allow, {S}. and van der {G}roen, {G}. and {S}hao, {Y}. and {J}acob, {M}. and {S}amuel, {N}. {M}. and de {R}ivera, {I}. {L}. and {N}go-{G}iang-{H}uong, {N}icole and {C}assol, {S}. and {A}lemnji, {G}. and {F}renkel, {L}. {M}.},
  editor = {},
  language = {{ENG}},
  abstract = {{O}bjective: {W}e evaluated the feasibility of the oligonucleotide ligation assay ({OLA}), a specific, sensitive, and economical ligase-based point mutation assay designed to detect {HIV}-1 drug-resistance mutations at 12 codons of {HIV}-1 subtype {B} pol, for potential use in resource-poor settings. {M}ethods: {S}pecimens from {HIV}-1-infected individuals collected by 7 international laboratories, including subtypes {A}, {B}, {C}, {D}, {F}, {G}, {J}, and recombinants {AE} and {AG}, were tested by the {OLA} developed for {HIV}-1 subtype {B}. {C}ommon polymorphisms that interfered with reactivity of the {OLA} were identified and modified probes designed and evaluated. {R}esults: 92.5% (2410) of 2604 codons in specimens from 217 individuals were successfully genotyped by the subtype {B} {OLA}. {A} high rate (range 8.3%-31.2%) of indeterminate results (negative {OLA} reaction for both mutant and wild type) was observed for 5 codons. {M}odified probes at reverse transcriptase codons 151 and 184 and protease codon 90 increased the rate of valid {OLA} to 96.1%. {C}onclusions: {T}he {OLA} designed for {HIV}-1 subtype {B} genotyped most pol codons in non-{B} subtypes from {A}sia and {A}frica but was improved by addition of several modified probes. {I}nternational laboratories experienced in molecular techniques were able to perform the {OLA}.},
  keywords = {{H}iv drug resistance ; {H}iv-1 non-b subtypes ; {R}esistance testing ; {O}ligonucleotide ligation assay ; {M}inor genotypes ; {P}oint mutation assay ; {D}ried blood spots},
  booktitle = {},
  journal = {{JAIDS} {J}ournal of {A}cquired {I}mmune {D}eficiency {S}yndromes},
  volume = {48},
  numero = {4},
  pages = {418--427},
  ISSN = {1525-4135},
  year = {2008},
  DOI = {10.1097/{QAI}.0b013e31817ed7d7},
  URL = {https://www.documentation.ird.fr/hor/fdi:010042699},
}