Varzakas T. H. auteur aut IRD Roussos Sevastianos auteur aut IRD Arvanitoyannis I. S. auteur aut IRD text journalArticle eng text/pdf born digital access This work presents the continuous production of fungal biomass and glucoamylase by solid state fermentation (SSF) in a counter-current reactor adapted for this purpose. Pre-germinated conidia of Aspergillus niger were used as an inoculum, and sugarcane bagasse, embedded with a nutritive solution, was the solid support. The solids residence time distribution (RTD) was carried out by feeding one impule of blue-coloured humidified bagasse and its RTD was fixed at 20 h. This study demonstrated that the values of the measured parameters (pH, moisture, biomass, glucoamylases production) were similar to those reported for batch SSF using the same solid support and micro-organism. A marked increase in biomass occurred from the progressive compartment (from compartment 1 to 9) into the reactor and the enzyme production was important (40 IU g(-1) dry exit solids). No mycelium damage or sporulation was observed by microscopy analysis. The above results confirmed that the continuous production of enzymes by SSF under no sterile conditions was successful. Inoculation with pre-germinated conidia shortened the processing time and allowed control of the age of the mycelium in each compartment. Aeration was accomplished by natural convection and moisture content had to be controlled. This process can be applied to the continuous production of fungal biomass and metabolites in SSF with industrial applications using environmental-friendly biotechnology. specialized Aspergillus niger Continuous-production Glucoamylases Pre-germinated conidia Solid state fermentation 084 43 7 1159-1168 2008 0950-5423 https://www.documentation.ird.fr/hor/fdi:010042655 10.1111/j.1365-2621.2007.01582.x 0950-5423 [F B010042655] https://www.documentation.ird.fr/hor/fdi:010042655 https://www.documentation.ird.fr/intranet/publi/2008/07/010042655.pdf Accès réservé (Intranet de l'IRD) IRD - Base Horizon / Pleins textes 2008-08-08 2017-08-23 fdi:010042655 fre