@article{fdi:010042651,
  title = {{S}ymbiotic properties of {M}ethylobacterium nodulans {ORS} 2060({T}) : {A} classic process for an atypical symbiont},
  author = {{R}enier, {A}deline and {J}ourand, {P}hilippe and {R}apior, {S}. and {P}oinsot, {V}. and {S}y, {A}bdoulaye and {D}reyfus, {B}ernard and {M}oulin, {L}ionel},
  editor = {},
  language = {{ENG}},
  abstract = {{S}ome legume species of the {C}rotalaria genus are specifically nodulated by methylotrophic bacteria belonging to the {M}ethylobacterium nodulans species. {T}he feature of this symbiotic bacterium is its ability to oxidize methanol, a property based on the presence of a methanol dehydrogenase enzyme. {D}espite a good knowledge of this property and its implication in symbiosis, the molecular dialogue between {M}. nodulans and crotalaria podocarpa leading to symbiosis is largely unknown, except the presence of a nod{A} nodulation gene in the genome of {M}. nodulans {ORS} 2060. {T}o investigate if {M}. nodulans {ORS} 2060 produces {N}od factors, molecules considered as the major bacteria-to-plant signals essential for the establishment of rhizobia-legume symbiosis, we identified and sequenced a nod{DABCUIJHQ} cluster from a genomic library of {ORS} 2060. {P}hylogenetic analyses of nod genes revealed that {M}. nodulans {ORS} 2060 form a branch together with {B}urkholderia tuberum {STM}678 and a strain of {M}ethylobacterium sp. (4-46) isolated from {L}otononis, and distinct from all the other rhizobia. {T}o analyse the regulation of {ORS} 2060 nod genes, we constructed a nod{A}-{L}ac{Z} promoter fusion to monitor the nod gene expression with various flavonoids. {T}he flavone apigenin was found to be the strongest inducer of nod gene expression in {M}. nodulans {ORS} 2060. {T}his latter flavonoid was used to induce {ORS} 2060, and {N}od factors were purified by high-performance liquid chromatography ({HPLC}) and further characterized by mass spectrometry. {O}ne major {N}od factor structure was identified as a pentamer of chitin substituted by {C}18:1 or {C}16:0 acyl chains on the non-reducing end and 6-{O}-sulphated on the other end, suggesting a classic symbiotic dialogue between {M}. nodulans and {C} podocarpa.},
  keywords = {{M}ethylobacterium nodulans ; {C}rotalaria spp ; {L}ipo-chito-oligosaccharide (lco) ; {N}od factor ; {F}lavonoids ; {A}pigenin ; {N}odulation genes ; {R}hizobia},
  booktitle = {},
  journal = {{S}oil {B}iology and {B}iochemistry},
  volume = {40},
  numero = {6},
  pages = {1404--1412},
  ISSN = {0038-0717},
  year = {2008},
  DOI = {10.1016/j.soilbio.2007.12.020},
  URL = {https://www.documentation.ird.fr/hor/fdi:010042651},
}