@article{fdi:010042624,
  title = {{E}xploring the {FL}-160-{CRP} gene family through sequence variability of the complement regulatory protein ({CRP}) expressed by the trypomastigote stage of {T}rypanosoma cruzi},
  author = {{M}athieu-{D}aud{\'e}, {F}ran{\c{c}}oise and {L}afay, {B}{\'e}n{\'e}dicte and {T}ouzet, {O}. and {L}elievre, {J}. and {P}arrado, {F}. and {B}osseno, {M}arie-{F}rance and {R}ojas, {A}. {M}. and {F}atha, {S}alima and {O}uaissi, {A}li and {B}reni{\`e}re, {S}imone {F}r{\'e}d{\'e}rique},
  editor = {},
  language = {{ENG}},
  abstract = {{T}he complement regulatory protein ({CRP}) of {T}rypanosoma cruzi is a surface glycoprotein which confers to the infectious trypomastigote forms a protection against the lytic activity of the host complement. {CRP} belongs to the large family of the trans-sialidase-like proteins and its sequence is highly similar to those of the flagellar {FL}-160 and chronic exoantigen proteins, encoded by a multigene family. {T}o further define the gene family encoding the {CRP}, we investigated the protein diversity among several strains of {T} cruzi through the sequencing of trypomastigote transcripts, and used a phylogenetic analysis based on the multiple alignment of these proteins with the top scoring sequences detected by a database sequence homology search. {I}ntrastrain variations in {CRP} sequences revealed the existence of several copies per strain. {T}he interstrain variability of {CRP} was consistent with the genetic subdivisions of {T} cruzi into lineages and discrete typing units. {T}he phylogenetic analysis based on a 227 amino acid alignment of {CRP} sequences with the 200 putative proteins retrieved from the protein databases (including the sequences from the {T} cruzi genome project) revealed that the {CRP} sequences clustered with the {FL}-160 proteins into a monophyletic group characterized by the presence of the 12 amino acid mimicry epitope that mimics nervous tissues. {T}he phylogeny did not differentiate between the {CRP} and the {FL}-160 proteins. {T}he identification of this group of {CRP}-like proteins and the high sequence similarity observed within it open up new prospects for the exploration of the localization, structure and function of these proteins and a better understanding of their involvement in key aspects of host-parasite interactions, such as the resistance to the complement. {T}his work provides also information for the {T} cruzi genome annotation of the trans-sialidase-like putative proteins.},
  keywords = {{T}rypanosoma cruzi ; complement regulatory protein ; {FL} 160 ; trans sialidase like proteins},
  booktitle = {},
  journal = {{I}nfection {G}enetics and {E}volution},
  volume = {8},
  numero = {3},
  pages = {258--266},
  ISSN = {1567-1348},
  year = {2008},
  DOI = {10.1016/j.meegid.2007.12.010},
  URL = {https://www.documentation.ird.fr/hor/fdi:010042624},
}