@article{fdi:010042614,
  title = {{G}enetic diversity and silencing suppression effects of {R}ice yellow mottle virus and the {P}1 protein - art. no. 55},
  author = {{S}ir{\'e}, {C}hristelle and {B}angratz {R}eyser, {M}artine and {F}argette, {D}enis and {B}rugidou, {C}hristophe},
  editor = {},
  language = {{ENG}},
  abstract = {{B}ackground: {PTGS} (post-transcriptional gene silencing) is used to counter pathogenic invasions, particularly viruses. {I}n return, many plant viruses produce proteins which suppress silencing directed against their {RNA}. {T}he diversity of silencing suppression at the species level in natural hosts is unknown. {R}esults: {W}e investigated the functional diversity of silencing suppression among isolates of the {A}frican {RYMV} ({R}ice yellow mottle virus) in rice. {T}he {RYMV}-{P}1 protein is responsible for cell-to-cell movement and is a silencing suppressor. {T}ransgenic gus-silencing rice lines were used to investigate intra-specific and serogroup silencing suppression diversity at two different levels: that of the virion and the {P}1 silencing suppressor protein. {O}ur data provide evidence that silencing suppression is a universal phenomenon for {RYMV} species. {H}owever, we found considerable diversity in their ability to suppress silencing which was not linked to {RYMV} phylogeny, or pathogenicity. {A}t the level of the silencing suppressor {P}1 alone, we found similar results to those previously found at the virion level. {I}n addition, we showed that cell-to-cell movement of {P}1 was crucial for the efficiency of silencing suppression. {M}utagenesis of {P}1 demonstrated a strong link between some amino acids and silencing suppression features with, one on the hand, the conserved amino acids {C}95 and {C}64 involved in cell-to-cell movement and the strength of suppression, respectively, and on the other hand, the non conserved {F}88 was involved in the strength of silencing suppression. {C}onclusion: {W}e demonstrated that intra-species diversity of silencing suppression is highly variable and by mutagenesis of {P}1 we established the first link between silencing suppression and genetic diversity. {T}hese results are potentially important for understanding virus-host interactions.},
  keywords = {},
  booktitle = {},
  journal = {{V}irology {J}ournal},
  volume = {5},
  numero = {},
  pages = {{NIL}_1--{NIL}_12},
  ISSN = {1743-422{X}},
  year = {2008},
  DOI = {10.1186/1743-422{X}-5-55},
  URL = {https://www.documentation.ird.fr/hor/fdi:010042614},
}