@article{fdi:010042561,
  title = {{D}ifferential gene expression in incipient species of {A}nopheles gambiae},
  author = {{C}assone, {B}. {J}. and {M}ouline, {K}. and {H}ahn, {M}. {W}. and {W}hite, {B}. {J}. and {P}ombi, {M}. and {S}imard, {F}r{\'e}d{\'e}ric and {C}ostantini, {C}arlo and {B}esansky, {N}. {J}.},
  editor = {},
  language = {{ENG}},
  abstract = {{A} speciation process is ongoing in the primary vector of malaria in {A}frica, {A}nopheles gambiae. {A}ssortatively mating incipient species known as the {M} and {S} forms differentially exploit larval breeding sites associated with different ecological settings. {H}owever, some ongoing gene flow between {M} and {S} limits significant genomic differentiation mainly to small centromere-proximal regions on chromosomes {X} and 2{L}, termed 'speciation islands' with the expectation that they contain the genes responsible for reproductive isolation. {A}s the speciation islands exhibit reduced recombination and low polymorphism, more detailed genetic analysis using fine-scale mapping is impractical. {W}e measured global gene expression differences between {M} and {S} using oligonucleotide microarrays, with the goal of identifying candidate genes that could be involved in this ongoing speciation process. {G}ene expression profiles were examined in two independent colonies of both forms at each of three developmental periods of interest: fourth instar larvae, virgin females, and gravid females. {P}atterns were validated on a subset of genes using quantitative real-time reverse transcription polymerase chain reaction of {RNA} samples from laboratory colonies and wild mosquitoes collected from {C}ameroon and {B}urkina {F}aso. {C}onsidered across all three developmental periods, differentially expressed genes represented similar to 1-2% of all expressed genes. {A}lthough disproportionately represented in the {X} speciation island, the vast majority of genes were located outside any speciation island. {C}ompared to samples from the other developmental periods, virgin females were characterized by more than twice as many differentially expressed genes, most notably those implicated in olfaction and potentially, mate recognition.},
  keywords = {{A}nopheles gambiae ; malaria vector ; microarray ; reverse ecology ; speciation},
  booktitle = {},
  journal = {{M}olecular {E}cology},
  volume = {17},
  numero = {10},
  pages = {2491--2504},
  ISSN = {0962-1083},
  year = {2008},
  DOI = {10.1111/j.1365-294{X}.2008.03774.x},
  URL = {https://www.documentation.ird.fr/hor/fdi:010042561},
}