Demaneche S. auteur aut IRD Sanguin H. auteur aut IRD Pote J. auteur aut IRD Navarro Elisabeth auteur aut IRD Bernillon D. auteur aut IRD Mavingui P. auteur aut IRD Wildi W. auteur aut IRD Vogel T. M. auteur aut IRD Simonet P. auteur aut IRD text journalArticle eng text/pdf born digital access Understanding the prevalence and polymorphism of antibiotic resistance genes in soil bacteria and their potential to be transferred horizontally is required to evaluate the likelihood and ecological (and possibly clinical) consequences of the transfer of these genes from transgenic plants to soil bacteria. In this study, we combined culture-dependent and -independent approaches to study the prevalence and diversity of bla genes in soil bacteria and the potential impact that a 110-successive-year culture of the transgenic Bt176 corn, which has a b/aTEM marker gene, could have had on the soil bacterial community. The bla gene encoding resistance to ampicillin belongs to the beta-lactam antibiotic family, which is widely used in medicine but is readily compromised by bacterial antibiotic resistance. Our results indicate that soil bacteria are naturally resistant to a broad spectrum of beta-lactam antibiotics, including the third cephalosporin generation, which has a slightly stronger discriminating effect on soil isolates than other cephalosporins. These high resistance levels for a wide range of antibiotics are partly due to the polymorphism of bla genes, which occur frequently among soil bacteria. The b/aTEM1 16 gene of the transgenic corn Bt176 investigated here is among those frequently found, thus reducing any risk of introducing a new bacterial resistance trait from the transgenic material. in addition, no significant differences were observed in bacterial antibiotic-resistance levels between transgenic and nontransgenic corn fields, although the bacterial populations were different. specialized antibiotic resistance GMO HGT 074 020 105 10 3957-3962 2008 0027-8424 https://www.documentation.ird.fr/hor/fdi:010042531 10.1073/pnas.0800072105 0027-8424 [F B010042531] https://www.documentation.ird.fr/hor/fdi:010042531 https://www.documentation.ird.fr/intranet/publi/2008/04/010042531.pdf Accès réservé (Intranet de l'IRD) IRD - Base Horizon / Pleins textes 2008-05-06 2020-06-12 fdi:010042531 fre