Horizon 1 1 17 ACL : Articles dans des revues avec comité de lecture répertoriées par l'AERES Molecular Breeding 105-113 breeding coffee Hemileia vastatrix leaf rust resistance marker assisted selection molecular marker 2008 fdi:010042527 ENG Molecular Breeding 1380-3743 CC:0002513214-0009 1 10.1007/s11032-007-9112-z https://www.documentation.ird.fr/hor/fdi:010042527 https://www.documentation.ird.fr/intranet/publi/2008/04/010042527.pdf 21 Horizon (IRD) Coffee leaf rust due to Hemileia vastatrix is one of the most serious diseases in Arabica coffee (Coffea arabica). A resistance gene (S-H(3)) has been transferred from C. liberica into C. arabica. The present work aimed at developing sequence-characterized genetic markers for leaf rust resistance. Linkage between markers and leaf rust resistance was tested by analysing two segregating populations, one F-2 population of 101 individuals and one backcross (BC2) population of 43 individuals, derived from a cross between a susceptible and a SH3-introgressed resistant genotype. A total of ten sequence-characterized genetic markers closely associated with the SH3 leaf rust resistance gene were generated. These included simple sequence repeats (SSR) markers, sequence-characterised amplified regions (SCAR) markers resulting from the conversion of amplified fragment length polymorphism (AFLP) markers previously identified and SCAR markers derived from end-sequences of bacterial artificial chromosome (BAC) clones. Those BAC clones were identified by screening of C. arabica genomic BAC library using a cloned AFLP-marker as probe. The markers we developed are easy and inexpensive to run, requiring one PCR step followed by gel separation. While three markers were linked in repulsion with the S-H(3) gene, seven markers were clustered in coupling around the S-H(3) gene. Notably, two markers appeared to co-segregate perfectly with the S-H(3) gene in the two plant populations analyzed. These markers are suitable for marker-assisted selection for leaf rust resistance and to facilitate pyramiding of the S-H(3) gene with other leaf rust resistance genes. 076