@article{fdi:010042527,
  title = {{D}evelopment of sequence characterized {DNA} markers linked to leaf rust ({H}emileia vastatrix) resistance in coffee ({C}offea arabica {L}.)},
  author = {{M}ah{\'e}, {L}aetitia and {C}ombes, {M}arie-{C}hristine and {V}arzea, {V}. {M}. {P}. and {G}uilhaumon, {C}laire and {L}ashermes, {P}hilippe},
  editor = {},
  language = {{ENG}},
  abstract = {{C}offee leaf rust due to {H}emileia vastatrix is one of the most serious diseases in {A}rabica coffee ({C}offea arabica). {A} resistance gene ({S}-{H}(3)) has been transferred from {C}. liberica into {C}. arabica. {T}he present work aimed at developing sequence-characterized genetic markers for leaf rust resistance. {L}inkage between markers and leaf rust resistance was tested by analysing two segregating populations, one {F}-2 population of 101 individuals and one backcross ({BC}2) population of 43 individuals, derived from a cross between a susceptible and a {SH}3-introgressed resistant genotype. {A} total of ten sequence-characterized genetic markers closely associated with the {SH}3 leaf rust resistance gene were generated. {T}hese included simple sequence repeats ({SSR}) markers, sequence-characterised amplified regions ({SCAR}) markers resulting from the conversion of amplified fragment length polymorphism ({AFLP}) markers previously identified and {SCAR} markers derived from end-sequences of bacterial artificial chromosome ({BAC}) clones. {T}hose {BAC} clones were identified by screening of {C}. arabica genomic {BAC} library using a cloned {AFLP}-marker as probe. {T}he markers we developed are easy and inexpensive to run, requiring one {PCR} step followed by gel separation. {W}hile three markers were linked in repulsion with the {S}-{H}(3) gene, seven markers were clustered in coupling around the {S}-{H}(3) gene. {N}otably, two markers appeared to co-segregate perfectly with the {S}-{H}(3) gene in the two plant populations analyzed. {T}hese markers are suitable for marker-assisted selection for leaf rust resistance and to facilitate pyramiding of the {S}-{H}(3) gene with other leaf rust resistance genes.},
  keywords = {breeding ; coffee ; {H}emileia vastatrix ; leaf rust resistance ; marker assisted selection ; molecular marker},
  booktitle = {},
  journal = {{M}olecular {B}reeding},
  volume = {21},
  numero = {1},
  pages = {105--113},
  ISSN = {1380-3743},
  year = {2008},
  DOI = {10.1007/s11032-007-9112-z},
  URL = {https://www.documentation.ird.fr/hor/fdi:010042527},
}