@article{fdi:010042475,
  title = {{PCR}-based karyotyping of {A}nopheles gambiae inversion 2{R}j identifies the {BAMAKO} chromosomal form - art. no. 133},
  author = {{C}oulibaly, {M}. {B}. and {P}ombi, {M}. and {C}aputo, {B}. and {N}wakanma, {D}. and {J}awara, {M}. and {K}onat{\'e}, {L}. and {D}ia, {I}. and {F}ofana, {A}. and {K}ern, {M}. and {S}imard, {F}r{\'e}d{\'e}ric and {C}onway, {D}. {J}. and {P}etrarca, {V}. and della {T}orre, {A}. and {T}raor{\'e}, {S}. and {B}esansky, {N}. {J}.},
  editor = {},
  language = {{ENG}},
  abstract = {{B}ackground: {T}he malaria vector {A}nopheles gambiae is polymorphic for chromosomal inversions on the right arm of chromosome 2 that segregate nonrandomly between assortatively mating populations in {W}est {A}frica. {O}ne such inversion, 2{R}j, is associated with the {BAMAKO} chromosomal form endemic to southern {M}ali and northern {G}uinea {C}onakry near the {N}iger {R}iver. {A}lthough it exploits a unique ecology and both molecular and chromosomal data suggest reduced gene flow between {BAMAKO} and other {A}. gambiae populations, no molecular markers exist to identify this form. {M}ethods: {T}o facilitate study of the {BAMAKO} form, a {PCR} assay for molecular karyotyping of 2{R}j was developed based on sequences at the breakpoint junctions. {T}he assay was extensively validated using more than 700 field specimens whose karyotypes were determined in parallel by cytogenetic and molecular methods. {A}s inversion 2{R}j also occurs in {SAVANNA} populations outside the geographic range of {BAMAKO}, samples were tested from {S}enegal, {C}ameroon and western {G}uinea {C}onakry as well as from {M}ali. {R}esults: {I}n southern {M}ali, where 2{R}j polymorphism in {SAVANNA} populations was very low and most of the 2{R}j homozygotes were found in {BAMAKO} karyotypes, the molecular and cytogenetic methods were almost perfectly congruent. {E}lsewhere agreement between the methods was much poorer, as the molecular assay frequently misclassified 2{R}j heterozygotes as 2{R}(+j) standard homozygotes. {C}onclusion: {M}olecular karyotyping of 2{R}j is robust and accurate on 2{R}(+j) standard and 2{R}j inverted homozygotes. {T}herefore, the proposed approach overcomes the lack of a rapid tool for identifying the {BAMAKO} form across developmental stages and sexes, and opens new perspectives for the study of {BAMAKO} ecology and behaviour. {O}n the other hand, the method should not be applied for molecular karyotyping of j-carriers within the {SAVANNA} chromosomal form.},
  keywords = {},
  booktitle = {},
  journal = {{M}alaria {J}ournal},
  volume = {6},
  numero = {},
  pages = {{NIL}_1--{NIL}_8},
  ISSN = {1475-2875},
  year = {2007},
  DOI = {10.1186/1475-2875-6-133},
  URL = {https://www.documentation.ird.fr/hor/fdi:010042475},
}