@article{fdi:010042462,
  title = {{T}rypanosoma brucei brucei induces alteration in the head proteome of the tsetse fly vector {G}lossina palpalis gambiensis},
  author = {{L}ef{\`e}vre, {T}hierry and {T}homas, {F}. and {R}avel, {S}ophie and {P}atrel, {D}elphine and {R}enault, {L}. and {L}e {B}ourligu, {L}. and {C}uny, {G}{\'e}rard and {B}iron, {D}avid},
  editor = {},
  language = {{ENG}},
  abstract = {{P}arasitic manipulations of host behaviour are known from a wide range of host-parasite associations. {H}owever, the understanding of these phenomena is far from complete and detailed investigation of their proximate causes is needed. {M}any studies report behavioural modifications, such as altered feeding rates in tsetse fly ({G}lossina) infected with the mature transmissible stage (i.e. metacyclic) of the trypanosomes. {H}ere, bidimensional (2{D}) gel electrophoresis and mass spectrometry were employed to analyse and compare the head proteome between four {G}lossina palpalis gambiensis categories (uninfected, refractory, mature infection, immature infection). {T}wenty-four protein spots specifically present or absent in the head of metacyclic-infected flies were observed. {T}hese protein spots were subsequently identified and functionally classified as glycolitic, neurotransmiter synthesis, signalling, molecular chaperone and transcriptional regulation proteins. {O}ur results indicate altered energy metabolism in the head of metacyclic-infected tsetse flies. {S}ome of the proteins identified, such as casein kinase 2 and jun kinase have previously been shown to play critical roles in apoptosis in insect neurones. {I}n addition, we found two pyridoxal-dependent decarboxylases (dopa decarboxylase and alpha methyldopa hypersensitive protein), suggesting a modification of serotonin and/or dopamine in the brain of metacyclic-infected tsetse flies. {O}ur data pave the way for future investigation of the alteration of the glossina central nervous system during infection by trypanosomes.},
  keywords = {{G}lossina palpalis gambiensis ; {T}rypanosoma brucei brucei ; behavioural changes ; proteomics ; mass spectrometry ; {MALDI}/{T}o{F} ; host parasite associations},
  booktitle = {},
  journal = {{I}nsect {M}olecular {B}iology},
  volume = {16},
  numero = {6},
  pages = {651--660},
  ISSN = {0962-1075},
  year = {2007},
  DOI = {10.1111/j.1365-2583.2007.00761.x},
  URL = {https://www.documentation.ird.fr/hor/fdi:010042462},
}