%0 Journal Article
%9 ACL : Articles dans des revues avec comité de lecture répertoriées par l'AERES
%A Poncet, Valérie
%A Hamon, Perla
%A De Saint Marc, M.B.S.
%A Bernard, T.
%A Hamon, Seger
%A Noirot, Michel
%T Base composition of Coffea AFLP sequences and their conservation within the genus
%D 2005
%L fdi:010042160
%G ENG
%J Journal of Heredity
%@ 0022-1503
%M CC:0002263092-0008
%N 1
%P 59-65
%R 10.1093/jhered/esi013
%U https://www.documentation.ird.fr/hor/fdi:010042160
%V 96
%W Horizon (IRD)
%X Amplified fragment length polymorphism (AFLP) is often used for genetic mapping and diversity analysis, but very little information is currently available on their sequence characteristics. Species-specific sequences were analyzed from a single Coffea genome (Coffea pseudozanguebariae) associated with clustered or nonclustered AFLP loci of known genetic position. Compared with the expressed sequence tag (EST) sequence composition, their AT content exhibited a bimodal distribution with AT-poor sequences corresponding mainly to putative coding sequences. AT-rich sequences, apart from the EST distribution, were usually clustered on the genetic map and might correspond to noncoding sequences. Conversion of these AFLP markers into sequence-characterized amplified region (SCAR) anchor markers allowed us to assess sequence conservation within Coffea species with respect to species relatedness.
%$ 076