de Carvalho M.D.S. auteur aut IRD Caldas E.D. auteur aut IRD Degallier Nicolas auteur aut IRD Vilarinhos P.D.T. auteur aut IRD De Souza L. auteur aut IRD Amelia M. auteur aut IRD Yoshizawa C. auteur aut IRD Knox M. B. auteur aut IRD De Oliveira C. auteur aut IRD text journalArticle eng text/pdf born digital access Objective: To study the susceptibility status of Aedes aegypti to the organophosphate insecticide temephos. Methods: Samples of Ae. aegypti larvae were obtained, using ovitraps, from eight cities of the Federal District, central Brazil, in 2000 and 2001. Larvae were submitted to the diagnostic dose of 0.012 mg/l temephos, as recommended by standard World Health Organization methodology. Field populations were tested in parallel with reference strains Rockefeller and DIVAL, from the Environmental Surveillance Directory (DIVAL) insectary. The concentration and purity of temephos solutions were verified by gas chromatography. Correlation calculations were performed using StatView - SAS Institute Inc., version 5. Student's t test was used for detecting differences in susceptibility, with significance levels of alpha=0.05. Results: In 2000, Ae. aegypti larvae populations from Taguatinga, Guara, and Nucleo Bandeirante showed resistance to temephos, with mortality ranging from 54.1 to 63.4%. The populations from Gama, Planaltina, and Sobradinho showed altered levels of susceptibility (mortality ranging from 83.6 to 92.8%). The population from Ceilandia was the only susceptible one, with 98% mortality. In 2001, all populations tested were resistant (44.4 to 66.4% mortality). No significant correlation was found between the susceptibility of populations and the distance between the cities of origin, or the amount of insecticide applied in the years preceding the study. Conclusions: Ae. aegypti susceptibility to temephos is changing in the Federal District. It is essential to continue monitoring the resistance of this vector to insecticides in order to ensure the efficiency of programs aimed at vector control and at the protection of human health. specialized aedes Temephos insecticide resistance vector control insect vectors BRESIL 052 38 5 623-629 2004 0034-8910 https://www.documentation.ird.fr/hor/fdi:010041526 0034-8910 [F B010041526] https://www.documentation.ird.fr/hor/fdi:010041526 https://www.documentation.ird.fr/intranet/publi/depot/0001/010041526.pdf Accès réservé (Intranet de l'IRD) IRD - Base Horizon / Pleins textes 2006-03-29 2024-03-22 fdi:010041526 fre