@article{fdi:010041491,
  title = {{R}ole of methylotrophy during symbiosis between {M}ethylobacterium nodulans and {C}rotalaria podocarpa},
  author = {{J}ourand, {P}hilippe and {R}enier, {A}deline and {R}apior, {S}. and {M}iana de {F}aria, {S}. and {P}rin, {Y}ves and {G}allana, {A}. and {G}iraud, {E}ric and {D}reyfus, {B}ernard},
  editor = {},
  language = {{ENG}},
  abstract = {{S}ome rare leguminous plants of the genus {C}rotalaria are specifically nodulated by the methylotrophic bacterium {M}ethylobacterium nodulans. {I}n this study, the expression and role of bacterial methylotrophy were investigated during symbiosis between {M}. nodulans, strain {ORS} 2060({T}), and its host legume, {C}rotalaria podocarpa. {U}sing lac{Z} fusion to the mxa{F} gene, we showed that the methylotroph genes are expressed in the root nodules, suggesting methylotrophic activity during symbiosis. {I}n addition, loss of the bacterial methylotrophic function significantly affected plant development. {I}ndeed, inoculation of {M}. nodulans nonmethylotroph mutants in {C}. podocarpa decreased the total root nodule number per plant up to 60%, decreased the whole-plant nitrogen fixation capacity up to 42%, and reduced the total dry plant biomass up to 46% compared with the wild-type strain. {I}n contrast, inoculation of the legume {C}. podocarpa with nonmethylotrophic mutants complemented with functional mxa genes restored the symbiotic wild phenotype. {T}hese results demonstrate the key role of methylotrophy during symbiosis between {M}. nodulans and {C}. podocarpa.},
  keywords = {methanol},
  booktitle = {},
  journal = {{M}olecular {P}lant {M}icrobe {I}nteractions},
  volume = {18},
  numero = {10},
  pages = {1061--1068},
  ISSN = {0894-0282},
  year = {2005},
  DOI = {10.1094/{MPMI}-18-1061},
  URL = {https://www.documentation.ird.fr/hor/fdi:010041491},
}