Chia Y.S. auteur aut IRD Badaut C. auteur aut IRD Ndam Nicaise auteur aut IRD Khattab A. auteur aut IRD Igonet S. auteur aut IRD Fievet Nadine auteur aut IRD Bentley G.A. auteur aut IRD Deloron Philippe auteur aut IRD Klinkert M.Q. auteur aut IRD text journalArticle eng text/pdf reformatted digital access A recombinant Duffy binding-like (DBL)-gamma domain from a previously identified placental isolate, 732, was expressed by use of the baculovirus/insect cell system and was purified in milligram quantities. The recombinant protein binds specifically to chondroitin sulfate A (CSA) and inhibits CSA binding by placental infected erythrocytes (IEs). Polyclonal antibodies raised against the domain recognized the surfaces of live IEs from CSA-adherent clinical placental isolates. These antibodies also abrogated the in vitro binding of IEs to CSA. The 732 DBL-3 gamma domain was specifically recognized by plasma from pregnant women but not by plasma from control subjects. In addition, the protein was, comparatively, significantly more reactive with plasma from women with infected placentas, strongly suggesting that the 732 DBL-3g domain carries preferentially IE-expressed immunogenic epitopes. High levels of plasma antibodies to the recombinant domain were associated with reduced placental parasite density. This is the first report of a recombinant DBL-g domain derived from a placental isolate that shows CSA-binding properties. specialized 052 192 7 1284-1293 2005 0022-1899 https://www.documentation.ird.fr/hor/fdi:010041471 10.1086/432918 0022-1899 [F B010041471] https://www.documentation.ird.fr/hor/fdi:010041471 https://horizon.documentation.ird.fr/exl-doc/pleins_textes/2024-12/010041471.pdf IRD - Base Horizon / Pleins textes 2006-03-29 2024-12-18 fdi:010041471 fre