@article{fdi:010041471,
  title = {{F}unctional and immunological characterization of a {D}uffy binding-like-gamma domain from {P}lasmodium falciparum erythrocyte membrane protein-1 expressed by a placental isolate},
  author = {{C}hia, {Y}.{S}. and {B}adaut, {C}. and {N}dam, {N}icaise and {K}hattab, {A}. and {I}gonet, {S}. and {F}ievet, {N}adine and {B}entley, {G}.{A}. and {D}eloron, {P}hilippe and {K}linkert, {M}.{Q}.},
  editor = {},
  language = {{ENG}},
  abstract = {{A} recombinant {D}uffy binding-like ({DBL})-gamma domain from a previously identified placental isolate, 732, was expressed by use of the baculovirus/insect cell system and was purified in milligram quantities. {T}he recombinant protein binds specifically to chondroitin sulfate {A} ({CSA}) and inhibits {CSA} binding by placental infected erythrocytes ({IE}s). {P}olyclonal antibodies raised against the domain recognized the surfaces of live {IE}s from {CSA}-adherent clinical placental isolates. {T}hese antibodies also abrogated the in vitro binding of {IE}s to {CSA}. {T}he 732 {DBL}-3 gamma domain was specifically recognized by plasma from pregnant women but not by plasma from control subjects. {I}n addition, the protein was, comparatively, significantly more reactive with plasma from women with infected placentas, strongly suggesting that the 732 {DBL}-3g domain carries preferentially {IE}-expressed immunogenic epitopes. {H}igh levels of plasma antibodies to the recombinant domain were associated with reduced placental parasite density. {T}his is the first report of a recombinant {DBL}-g domain derived from a placental isolate that shows {CSA}-binding properties.},
  keywords = {},
  booktitle = {},
  journal = {{J}ournal of {I}nfectious {D}iseases},
  volume = {192},
  numero = {7},
  pages = {1284--1293},
  ISSN = {0022-1899},
  year = {2005},
  DOI = {10.1086/432918},
  URL = {https://www.documentation.ird.fr/hor/fdi:010041471},
}