@article{fdi:010040999,
  title = {{A}ndrogenic potential in coconut ({C}ocos nucifera {L}.)},
  author = {{P}erera, {P}. {I}. {P}. and {H}ocher, {V}al{\'e}rie and {V}erdeil, {J}. {L}. and {B}andupriya, {H}. {D}. {D}. and {Y}akandawala, {D}. {M}. {D}. and {W}eerakoon, {L}. {K}.},
  editor = {},
  language = {{ENG}},
  abstract = {{C}onditions for induction of androgenesis in coconut cv. {S}ri {L}anka {T}all were studied. {A}nthers collected from inflorescences at four maturity stages were given heat (38 degrees {C}) or cold (4 degrees {C}) pretreatments for 1, 3, 6 and 14 days, either prior to or post inoculation. {T}hree different basal media and different anther densities were also tested. {A}ndrogenesis was observed only in anthers collected from inflorescences 3 weeks before splitting ({WBS}) and after a heat pretreatment at 38 degrees {C} for 6 days. {M}odified {E}euwens {Y}-3 liquid medium supplemented with 100 mu {M} 2,4-dichlorophenoxyacetic acid (2,4-{D}), 0.1% activated charcoal and 9% sucrose was effective in inducing an androgenic response. {T}he lowest anther density tested, 10 per petri plate, was found to be the optimal density. {W}hen androgenic calli or embryos were subcultured to {Y}-3 medium containing 66 mu {M} 2,4-{D}, followed by transfer to {Y}-3 medium without plant growth regulators and finally to {Y}-3 medium containing 5 mu {M} 6-benzyladenine ({BA}) and 0.35 mu {M} gibberellic acid ({GA}(3)), plantlets regenerated at a frequency of 7%. {H}istological study indicated that the calli and embryos originated from the inner tissues of the anthers. {P}loidy analysis of calli and embryos showed that they were haploid. {T}his is the first report of successful androgenesis yielding haploid plants from coconut anthers.},
  keywords = {},
  booktitle = {},
  journal = {{P}lant {C}ell {T}issue and {O}rgan {C}ulture},
  volume = {92},
  numero = {3},
  pages = {293--302},
  ISSN = {0167-6857},
  year = {2008},
  DOI = {10.1007/s11240-008-9337-5},
  URL = {https://www.documentation.ird.fr/hor/fdi:010040999},
}