Schrevel J. auteur aut IRD Asfaux Foucher G. auteur aut IRD Hopkins J. M. auteur aut IRD Robert Vincent auteur aut IRD Bourgouin C. auteur aut IRD Prensier G. auteur aut IRD Bannister L. H. auteur aut IRD text journalArticle eng text/pdf born digital access Oocysts from Anopheles stephensi mosquitoes fed on murine blood infected with Plasmodium berghei berghei, were fixed for electron microscopy 6-12 days post-feeding. Ultrastructural analysis focused on Golgi-related trafficking pathways for rhoptry and microneme formation during sporogony. A small Golgi complex of 1-3 cisternae is formed close to the spindle pole body from coated vesicles budded from the nuclear envelope which is confluent with the endoplasmic reticulum. Rhoptries begin as small spheroidal bodies apparently formed by fusion of Golgi-derived vesicles, lengthening to 3-4 mu m, and increasing in number to 4 per sporozoite. Ultrastructural data indicate the presence of a novel mechanism for vesicle transport between the Golgi complex and rhoptries along a longitudinal 30 nm - thick fibre (rootlet fibre or tigelle). Filamentous links between vesicles and rootlet indicate that this is a previously undescribed vesicle transport organelle. Genesis of micronernes occurs late in bud maturation and starts as spheroidal dense-cored vesicles (pro-micronemes), transforming to their mature bottle-like shape as they move apically. Filamentous links also occur between micronernes and subpellicular microtubules, indicating that as in merozoites, micronemes are trafficked actively along these structures. specialized 052 020 135 1 1-12 2008 0031-1820 https://www.documentation.ird.fr/hor/fdi:010040996 10.1017/S0031182007003629 0031-1820 [F B010040996] https://www.documentation.ird.fr/hor/fdi:010040996 https://www.documentation.ird.fr/intranet/publi/2008/03/010040996.pdf Accès réservé (Intranet de l'IRD) IRD - Base Horizon / Pleins textes 2008-03-31 2017-08-23 fdi:010040996 fre