%0 Journal Article
%9 ACL : Articles dans des revues avec comité de lecture répertoriées par l'AERES
%A Schrevel, J.
%A Asfaux Foucher, G.
%A Hopkins, J. M.
%A Robert, Vincent
%A Bourgouin, C.
%A Prensier, G.
%A Bannister, L. H.
%T Vesicle trafficking during sporozoite development in Plasmodium berghei : ultrastructural evidence for a novel trafficking mechanism
%D 2008
%L fdi:010040996
%G ENG
%J Parasitology
%@ 0031-1820
%M CC:0002531515-0001
%N 1
%P 1-12
%R 10.1017/S0031182007003629
%U https://www.documentation.ird.fr/hor/fdi:010040996
%> https://www.documentation.ird.fr/intranet/publi/2008/03/010040996.pdf
%V 135
%W Horizon (IRD)
%X Oocysts from Anopheles stephensi mosquitoes fed on murine blood infected with Plasmodium berghei berghei, were fixed for electron microscopy 6-12 days post-feeding. Ultrastructural analysis focused on Golgi-related trafficking pathways for rhoptry and microneme formation during sporogony. A small Golgi complex of 1-3 cisternae is formed close to the spindle pole body from coated vesicles budded from the nuclear envelope which is confluent with the endoplasmic reticulum. Rhoptries begin as small spheroidal bodies apparently formed by fusion of Golgi-derived vesicles, lengthening to 3-4 mu m, and increasing in number to 4 per sporozoite. Ultrastructural data indicate the presence of a novel mechanism for vesicle transport between the Golgi complex and rhoptries along a longitudinal 30 nm - thick fibre (rootlet fibre or tigelle). Filamentous links between vesicles and rootlet indicate that this is a previously undescribed vesicle transport organelle. Genesis of micronernes occurs late in bud maturation and starts as spheroidal dense-cored vesicles (pro-micronemes), transforming to their mature bottle-like shape as they move apically. Filamentous links also occur between micronernes and subpellicular microtubules, indicating that as in merozoites, micronemes are trafficked actively along these structures.
%$ 052 ; 020