@article{fdi:010040923,
  title = {{T}herapeutic efficacy of sulfadoxine-pyrimethamine and the prevalence of molecular markers of resistance in under 5-year olds in {B}razzaville, {C}ongo},
  author = {{N}dounga, {M}. and {T}ahar, {R}achida and {B}asco, {L}eonardo and {C}asimiro, {P}. {N}. and {M}alonga, {D}. {A}. and {N}toumi, {F}.},
  editor = {},
  language = {{ENG}},
  abstract = {{OBJECTIVE} {T}o test the efficacy of sulfadoxine-pyremethamine ({SP}) monotherapy and establish the prevalence of mutations in dhfr and dhps in {B}razzaville, {C}ongo. {METHOD} {W}e recruited 97 patients aged 6-59 months with uncomplicated malaria who attended {T}enrikyo public health {C}entre. {E}ighty-three were followed until day 28. {SP} efficacy was determined by the {WHO} 28-day test and analysis of mutations in the {P}lasmodium falciparum dihydrofolate reductase (pfdhfr) and clihydropteroate synthase (pfhbps) genes. {RESULT}s {T}here were seven (8.4%) early treatment failures, 23 late treatment failures (27.7%), nine (10.8%) late parasitological failures and 44 (53%) adequate clinical and parasitological responses ({ACPR}). {A}fter polymerase chain reaction ({PCR}) analysis of 64 available samples, the corrected results there were 44 (68.8%) {ACPR} and 19 recrudescent cases (31.2%). {A}pproximately, 97.5% of samples bore the {A}sn51lle mutation, 66.2% the {C}ys59{A}rg mutation and 98.8% the {S}er108{A}sn mutation. {M}utations of dhps at positions 437 ({A}la-{G}ly) and 436 ({S}er-{A}la) were found in 85% and 12.5% of samples. {Q}uadruple mutations (pfdhfr triple mutations in codons 51, 59 and 108+ pfdhps mutation in 437) were found in 42 samples (52.5%) and associated with treatment failures. {CONCLUSION} {T}his high level of treatment failures and mutations in both genes calls for the urgent application of the new policy for malaria treatment to delay the spread of {SP} resistance.},
  keywords = {plasmodium falciparum ; sulfadoxine pyrimethamine ; efficacy ; treatment failures ; dihydro folate ; reductase ; dihydropteroate synthase ; {B}razzaville ; {CONGO} ; {BRAZZAVILLE}},
  booktitle = {},
  journal = {{T}ropical {M}edicine and {I}nternational {H}ealth},
  volume = {12},
  numero = {10},
  pages = {1164--1171},
  ISSN = {1360-2276},
  year = {2007},
  DOI = {10.1111/j.1365-3156.2007.01904.x},
  URL = {https://www.documentation.ird.fr/hor/fdi:010040923},
}