Obertello Mariana auteur aut IRD Wall L. auteur aut IRD Laplaze Laurent auteur aut IRD Nicole Michel auteur aut IRD Auguy Florence auteur aut IRD Gherbi Hassen auteur aut IRD Bogusz Didier auteur aut IRD Franche Claudine auteur aut IRD text journalArticle eng text/pdf reformatted digital access cgMT1 is a metallothionein (MT)-like gene that was isolated from a cDNA library of young nitrogen-fixing nodules resulting from the symbiotic interaction between Frankia spp. and the actinorhizal tree Casuarina glauca. cgMT1 is highly transcribed in the lateral roots and nitrogen-fixing cells of actinorhizal nodules; it encodes a class I type 1 MT. To obtain insight into the function of cgMT1, we studied factors regulating the expression of the MT promoter region (PcgMT1) using a beta-glucuronidase (gus) fusion approach in transgenic plants of Arabidopsis thaliana. We found that copper, zinc, and cadmium ions had no significant effect on the regulation of PcgMT1-gus expression whereas wounding and H2O2 treatments led to an increase in reporter gene activity in transgenic leaves. Strong PcgMT1-gus expression also was observed when transgenic plants were inoculated with a virulent strain of the bacterial pathogen Xanthomonas campestris pv. campestris. Transgenic Arabidopsis plants expressing cgMT1 under the control of the constitutive 35S promoter were characterized by reduced accumulation of H2O2 when leaves were wounded and by increased susceptibility to the bacterial pathogen X. campestris. These results suggest that cgMT1 could play a role during the oxidative response linked to biotic and abiotic stresses. specialized 084 20 10 1231-1240 2007 0894-0282 https://www.documentation.ird.fr/hor/fdi:010040911 10.1094/MPMI-20-10-1231 0894-0282 [F B010040911] https://www.documentation.ird.fr/hor/fdi:010040911 https://horizon.documentation.ird.fr/exl-doc/pleins_textes/2024-12/010040911.pdf IRD - Base Horizon / Pleins textes 2008-02-22 2024-12-24 fdi:010040911 fre