@article{fdi:010040911, title = {{F}unctional analysis of the metallothionein gene cg{MT}1 isolated from the actinorhizal tree {C}asuarina glauca}, author = {{O}bertello, {M}ariana and {W}all, {L}. and {L}aplaze, {L}aurent and {N}icole, {M}ichel and {A}uguy, {F}lorence and {G}herbi, {H}assen and {B}ogusz, {D}idier and {F}ranche, {C}laudine}, editor = {}, language = {{ENG}}, abstract = {cg{MT}1 is a metallothionein ({MT})-like gene that was isolated from a c{DNA} library of young nitrogen-fixing nodules resulting from the symbiotic interaction between {F}rankia spp. and the actinorhizal tree {C}asuarina glauca. cg{MT}1 is highly transcribed in the lateral roots and nitrogen-fixing cells of actinorhizal nodules; it encodes a class {I} type 1 {MT}. {T}o obtain insight into the function of cg{MT}1, we studied factors regulating the expression of the {MT} promoter region ({P}cg{MT}1) using a beta-glucuronidase (gus) fusion approach in transgenic plants of {A}rabidopsis thaliana. {W}e found that copper, zinc, and cadmium ions had no significant effect on the regulation of {P}cg{MT}1-gus expression whereas wounding and {H}2{O}2 treatments led to an increase in reporter gene activity in transgenic leaves. {S}trong {P}cg{MT}1-gus expression also was observed when transgenic plants were inoculated with a virulent strain of the bacterial pathogen {X}anthomonas campestris pv. campestris. {T}ransgenic {A}rabidopsis plants expressing cg{MT}1 under the control of the constitutive 35{S} promoter were characterized by reduced accumulation of {H}2{O}2 when leaves were wounded and by increased susceptibility to the bacterial pathogen {X}. campestris. {T}hese results suggest that cg{MT}1 could play a role during the oxidative response linked to biotic and abiotic stresses.}, keywords = {}, booktitle = {}, journal = {{M}olecular {P}lant {M}icrobe {I}nteractions}, volume = {20}, numero = {10}, pages = {1231--1240}, ISSN = {0894-0282}, year = {2007}, DOI = {10.1094/{MPMI}-20-10-1231}, URL = {https://www.documentation.ird.fr/hor/fdi:010040911}, }