Mahdhi M. auteur aut IRD Nzoué Angèle auteur aut IRD Gueye Fatou auteur aut IRD Merabet Chahinez auteur aut IRD Lajudie de Philippe auteur aut IRD Mars M. auteur aut IRD text journalArticle eng text/pdf reformatted digital access Genista saharae, indigenous of Sahara, is a spontaneous shrub that plays an important ecological role for the preservation and fertility of poor and eroded soils. This legume has not been examined for its root nodule bacteria. The taxonomic diversity of bacteria from root nodules of G. saharae growing in the infra-arid region of Tunisia was investigated. A total of 28 bacterial strains isolated from root nodules of G. saharae grown in Tunisian soil were characterized using a polyphasic approach including phenotypic characteristics, PCR-RFLP of 16S rDNA and 16S rRNA gene sequencing. It was found that new isolates are diverse and affiliated to Ensifer (75%), Rhizobium (10%) and Phyllobacterium (15%). The Phyllobacterium isolates lacked the capacity for nodule formation on this plant. Genista saharae formed nodules with diverse rhizobia in Tunisian soils. Furthermore, our results support the presence of non-nodulating commensal strains (Phyllobacterium) in legumes nodule. This study is the first report on the characterization of G. saharae microsymbionts in Tunisia. specialized 16S rDNA sequencing Genista saharae legume nodule bacteria RFLP Tunisia 084 45 6 604-609 2007 0266-8254 https://www.documentation.ird.fr/hor/fdi:010040902 10.1111/j.1472-765X.2007.02233.x 0266-8254 [F B010040902] https://www.documentation.ird.fr/hor/fdi:010040902 https://horizon.documentation.ird.fr/exl-doc/pleins_textes/2024-12/010040902.pdf IRD - Base Horizon / Pleins textes 2008-02-22 2024-12-24 fdi:010040902 fre