%0 Journal Article
%9 ACL : Articles dans des revues avec comité de lecture répertoriées par l'AERES
%A Gonzalez Arnao, M. T.
%A Panta, A.
%A Roca, W. M.
%A Escobar, R. H.
%A Engelmann, Florent
%T Development and large scale application of cryopreservation techniques for shoot and somatic embryo cultures of tropical crops
%D 2008
%L fdi:010040897
%G ENG
%J Plant Cell Tissue and Organ Culture
%@ 0167-6857
%K cryopreservation ; shoot tips ; somatic embryos ; vitrification ; genebank
%M ISI:000251373300001
%N 1
%P 1-13
%R 10.1007/s11240-007-9303-7
%U https://www.documentation.ird.fr/hor/fdi:010040897
%> https://www.documentation.ird.fr/intranet/publi/2008/01/010040897.pdf
%V 92
%W Horizon (IRD)
%X Shoot-tips and somatic embryos are the explants of choice for the in vitro long-term storage of ex situ plant genetic resources in liquid nitrogen. Cryopreservation of organized structures has significantly progressed, especially for species of tropical origin, with the development of several vitrification-based procedures such as encapsulation-dehydration, vitrification and droplet-vitrification approaches. They have allowed improvements in survival and recovery after cryopreservation compared with conventional crystallization-based protocols, proving their effectiveness for large scale application with embryos and shoot-tips of different plants. This review addresses the main physical and technological aspects involved in plant cryopreservation methods, illustrating the development of research with three cases: citrus, cassava and potato. These studies demonstrate how cryopreservation strategies are increasingly applied for their successful employment in the genebanks.
%$ 076