@article{fdi:010040897,
  title = {{D}evelopment and large scale application of cryopreservation techniques for shoot and somatic embryo cultures of tropical crops},
  author = {{G}onzalez {A}rnao, {M}. {T}. and {P}anta, {A}. and {R}oca, {W}. {M}. and {E}scobar, {R}. {H}. and {E}ngelmann, {F}lorent},
  editor = {},
  language = {{ENG}},
  abstract = {{S}hoot-tips and somatic embryos are the explants of choice for the in vitro long-term storage of ex situ plant genetic resources in liquid nitrogen. {C}ryopreservation of organized structures has significantly progressed, especially for species of tropical origin, with the development of several vitrification-based procedures such as encapsulation-dehydration, vitrification and droplet-vitrification approaches. {T}hey have allowed improvements in survival and recovery after cryopreservation compared with conventional crystallization-based protocols, proving their effectiveness for large scale application with embryos and shoot-tips of different plants. {T}his review addresses the main physical and technological aspects involved in plant cryopreservation methods, illustrating the development of research with three cases: citrus, cassava and potato. {T}hese studies demonstrate how cryopreservation strategies are increasingly applied for their successful employment in the genebanks.},
  keywords = {cryopreservation ; shoot tips ; somatic embryos ; vitrification ; genebank},
  booktitle = {},
  journal = {{P}lant {C}ell {T}issue and {O}rgan {C}ulture},
  volume = {92},
  numero = {1},
  pages = {1--13},
  ISSN = {0167-6857},
  year = {2008},
  DOI = {10.1007/s11240-007-9303-7},
  URL = {https://www.documentation.ird.fr/hor/fdi:010040897},
}