%0 Journal Article
%9 ACL : Articles dans des revues avec comité de lecture répertoriées par l'AERES
%A Chomont, N.
%A Hocini, H.
%A Gody, J. C.
%A Bouhlal, H.
%A Becquart, Pierre
%A Krief Bouillet, C.
%A Kazatchkine, M.
%A Belec, L.
%T Neutralizing monoclonal antibodies to human immunodeficiency virus type 1 do not inhibit viral transcytosis through mucosal epithelial cells
%D 2007
%L fdi:010040892
%G ENG
%J Virology
%@ 0042-6822
%K HIV 1 ; HEC 1 ; transcytosis ; neutralization
%M ISI:000251698800004
%N 2
%P 246-254
%R 10.1016/j.virol.2007.09.006
%U https://www.documentation.ird.fr/hor/fdi:010040892
%> https://www.documentation.ird.fr/intranet/publi/2008/01/010040892.pdf
%V 370
%W Horizon (IRD)
%X HIV-1 transcytosis has been proposed as a potential mechanism allowing the virus to cross the epithelium during mucosal transmission. Epitopes of the HIV-1 envelope involved in this process have not been identified yet. Here, we assessed a large panel of HIV neutralizing antibodies recognizing well-characterized epitopes of the HIV-1 envelope for their ability to block HIV-1 transcytosis across a confluent epithelial monolayer. We found that all of the 13 HIV-1-specific monoclonal antibodies tested in the present study, including the three broadly neutralizing antibodies 2F5. 2G12 and lgG1bI2, lacked the ability to inhibit transcytosis of cell-free and cell-associated R5- as X4-tropic HIV-1 across a tight and polarized monolayer of HEC-1 epithelial cells. In contrast, anti-gp 160 polyclonal antibodies purified from serum or breast milk of HIV-1-infected individuals potently inhibited HIV-1 transcytosis. Furthermore, polymeric S-IgA exhibited similar ability to inhibit transcytosis compared to IgG despite their lower anti-gp 160 specific activity. Together, these results demonstrate that the major neutralizing envelope epitopes of HIV-1 are not involved in HIV-1 transcytosis, and suggest that surface agglutination of virus particles may participate to the blocking effect observed with both polyclonal and polymeric anti-gp 160 immunoglobulins.
%$ 052