Postec A. auteur aut IRD Lesongeur F. auteur aut IRD Pignet P. auteur aut IRD Ollivier Bernard auteur aut IRD Querellou J. auteur aut IRD Godfroy A. auteur aut IRD text journalArticle eng text/pdf born digital access The prokaryotic diversity of culturable thermophilic communities of deep-sea hydrothermal chimneys was analysed using a continuous enrichment culture performed in a gas-lift bioreactor, and compared to classical batch enrichment cultures in vials. Cultures were conducted at 60 degrees C and pH 6.5 using a complex medium containing carbohydrates, peptides and sulphur, and inoculated with a sample of a hydrothermal black chimney collected at the Rainbow field, Mid-Atlantic Ridge, at 2,275 m depth. To assess the relevance of both culture methods, bacterial and archaeal diversity was studied using cloning and sequencing, DGGE, and whole-cell hybridisation of 16S rRNA genes. Sequences of heterotrophic microorganisms belonging to the genera Marinitoga, Thermosipho, Caminicella (Bacteria) and Thermococcus (Archaea) were obtained from both batch and continuous enrichment cultures while sequences of the autotrophic bacterial genera Deferribacter and Thermodesulftitator were only detected in the continuous bioreactor culture. It is presumed that over time constant metabolite exchanges will have occurred in the continuous enrichment culture enabling the development of a more diverse prokaryotic community. In particular, CO2 and H-2 produced by the heterotrophic population would support the growth of autotrophic populations. Therefore, continuous enrichment culture is a useful technique to grow over time environmentally representative microbial communities and obtain insights into prokaryotic species interactions that play a crucial role in deep hydrothermal environments. specialized microbial diversity deep sea hydrothermal vent continuous enrichment cultures bioreactor thermophiles 16S rRNA gene metabolic interactions 084 11 6 747-757 2007 1431-0651 https://www.documentation.ird.fr/hor/fdi:010040887 10.1007/s00792-007-0092-z 1431-0651 [F B010040887] https://www.documentation.ird.fr/hor/fdi:010040887 https://www.documentation.ird.fr/intranet/publi/2008/01/010040887.pdf Accès réservé (Intranet de l'IRD) IRD - Base Horizon / Pleins textes 2008-02-22 2017-08-23 fdi:010040887 fre