@article{fdi:010040887,
  title = {{C}ontinuous enrichment cultures : insights into prokaryotic diversity and metabolic interactions in deep-sea vent chimneys},
  author = {{P}ostec, {A}. and {L}esongeur, {F}. and {P}ignet, {P}. and {O}llivier, {B}ernard and {Q}uerellou, {J}. and {G}odfroy, {A}.},
  editor = {},
  language = {{ENG}},
  abstract = {{T}he prokaryotic diversity of culturable thermophilic communities of deep-sea hydrothermal chimneys was analysed using a continuous enrichment culture performed in a gas-lift bioreactor, and compared to classical batch enrichment cultures in vials. {C}ultures were conducted at 60 degrees {C} and p{H} 6.5 using a complex medium containing carbohydrates, peptides and sulphur, and inoculated with a sample of a hydrothermal black chimney collected at the {R}ainbow field, {M}id-{A}tlantic {R}idge, at 2,275 m depth. {T}o assess the relevance of both culture methods, bacterial and archaeal diversity was studied using cloning and sequencing, {DGGE}, and whole-cell hybridisation of 16{S} r{RNA} genes. {S}equences of heterotrophic microorganisms belonging to the genera {M}arinitoga, {T}hermosipho, {C}aminicella ({B}acteria) and {T}hermococcus ({A}rchaea) were obtained from both batch and continuous enrichment cultures while sequences of the autotrophic bacterial genera {D}eferribacter and {T}hermodesulftitator were only detected in the continuous bioreactor culture. {I}t is presumed that over time constant metabolite exchanges will have occurred in the continuous enrichment culture enabling the development of a more diverse prokaryotic community. {I}n particular, {CO}2 and {H}-2 produced by the heterotrophic population would support the growth of autotrophic populations. {T}herefore, continuous enrichment culture is a useful technique to grow over time environmentally representative microbial communities and obtain insights into prokaryotic species interactions that play a crucial role in deep hydrothermal environments.},
  keywords = {microbial diversity ; deep sea hydrothermal vent ; continuous enrichment cultures ; bioreactor ; thermophiles ; 16{S} r{RNA} gene ; metabolic interactions},
  booktitle = {},
  journal = {{E}xtremophiles},
  volume = {11},
  numero = {6},
  pages = {747--757},
  ISSN = {1431-0651},
  year = {2007},
  DOI = {10.1007/s00792-007-0092-z},
  URL = {https://www.documentation.ird.fr/hor/fdi:010040887},
}