@article{fdi:010040798,
  title = {{C}ryopreservation of somatic embryos of paradise tree ({M}elia azedarach {L}.)},
  author = {{S}cocchi, {A}. and {V}ila, {S}. and {M}roginski, {L}. and {E}ngelmann, {F}lorent},
  editor = {},
  language = {{ENG}},
  abstract = {{I}n paradise tree ({M}elia azedarach {L}.), immature zygotic embryos sampled from immature fruits are the starting material for the production of somatic embryos. {T}hese somatic embryos are employed for freezing experiments. {I}mmature fruits could be stored at 25 degrees {C} for up to 80 days without impairing the embryogenic potential of zygotic embryos, which represents a four-fold increase in immature fruit storage duration, compared with previous studies. {A}mong the three cryopreservation techniques tested for freezing paradise tree somatic embryos, namely desiccation, encapsulation-dehydration and pregrowth-dehydration, only encapsulation-dehydration and pregrowth-dehydration led to successful results. {T}he optimal protocol was the following: i) somatic embryos (encapsulated or not) pretreated in liquid {M}urashige and {S}koog medium with daily increasing sucrose concentration (0.5 {M}/0.75 {M}/1.0 {M}); ii) dehydrated with silica gel to 21 - 26% moisture content (fresh weight basis), for encapsulation-dehydration, or to 19% moisture content, for pregrowth-dehydration; iii) frozen at 1 degrees {C}/min from 20 degrees {C} to -30 degrees {C} with a programmable freezing apparatus; iv) rapid immersion in liquid nitrogen. {T}he highest recovery achieved was 36% with encapsulation-dehydration and 30% with pregrowth-dehydration. {R}egrowth of frozen embryos was direct in most cases, as secondary embryogenesis originating from the root pole was observed on only around 10% of cryopreserved somatic embryos. {P}lants recovered from cryopreserved embryos presented the same phenotypic traits as non-frozen control plants.},
  keywords = {immature fruits ; zygotic embryos ; encapsulation dehydration ; pregrowth dehydration ; sucrose ; desiccation ; two step freezing},
  booktitle = {},
  journal = {{C}ryoletters},
  volume = {28},
  numero = {4},
  pages = {281--290},
  ISSN = {0143-2044},
  year = {2007},
  URL = {https://www.documentation.ird.fr/hor/fdi:010040798},
}