@article{fdi:010040792,
  title = {{C}haracterization of the anti-{L}eishmania effect induced by cisplatin, an anticancer drug},
  author = {{T}avares, {J}oana and {O}uaissi, {M}. and {O}uaissi, {A}li and {C}ordeiro {D}a {S}ilva, {A}.},
  editor = {},
  language = {{ENG}},
  abstract = {{T}he cis-diamminedichloroplatinum({II}), known as cis-{DDP} or cisplatin is a widely used drug in cancer chemotherapy. {A}lthough a recent study has shown the anti-{L}eishmania activity of some cis-{DDP} derivatives, the cytotoxic properties were measured only on promastigotes, the insect vector form of the parasite. {I}n this study the effect of cis-{DDP} on promastigotes and amastigotes, the vertebrate stage of the parasite is reported. {T}he {IC}50, determined by flow cytometry, after 72 {I}t of drug incubation was four times higher, 7.73 +/- 1.03 mu {M} in the case of promastigotes compared to axenic amastigotes, 1.88 +/- 0.10 mu {M}. {I}n intracellular amastigotes the {IC}50, determined by counting the parasite index was 1.85 +/- 0.22 mu {M}. {B}y using flow cytometry, two patterns of cell cycle changes was observed: cis-{DDP} treated promastigotes and amastigotes accumulated in {S} phase and {G}2 phase, respectively. {T}he cis-{DDP} response was also found to involve an "apoptosis-like" death of both promastigotes and amastigotes. {H}owever, {DNA} fragmentation was only detected in promastigote forms. {I}n contrast mitochondrial transmembrane potential loss was observed for both stages of the parasite. {U}pon incubation of parasites with the drug an increase on {GSH} and {GSSG} levels and reactive oxygen species could be detected in the case of promastigote. {M}oreover, a slight increase of {GSH} level was detected on amastigote form. {T}aken together, these observations indicate that amastigotes are more sensitive to cis-{DDP} when compared to promastigotes. {H}owever, the signaling pathways leading to cell death could be different.},
  keywords = {cisplatin ; {L}eishmania infantum ; anticancer drug ; cell death},
  booktitle = {},
  journal = {{A}cta {T}ropica},
  volume = {103},
  numero = {2},
  pages = {133--141},
  ISSN = {0001-706{X}},
  year = {2007},
  DOI = {10.1016/j.actatropica.2007.05.017},
  URL = {https://www.documentation.ird.fr/hor/fdi:010040792},
}