@article{fdi:010040791,
  title = {{M}olecular epidemiology of malaria in {C}ameroon {XXVII}. {C}linical and parasitological response to sulfadoxine-pyrimethamine treatment and {P}lasmodium falciparum dihydrofolate reductase and dihydropteroate synthase alleles in {C}ameroonian children},
  author = {{T}ahar, {R}achida and {B}asco, {L}eonardo},
  editor = {},
  language = {{ENG}},
  abstract = {{T}he rapidly changing epidemiology of anti folate-resistant {P}lasmodium falciparum in {A}frica requires monitoring. {T}he present study was designed to assess the degree of association between the clinical and parasitological response to sulfadoxine-pyrimethamine and allelic combinations of dihydrofolate reductase (dhfr) and dihydropteroate synthase (dhps) genes. {O}f 357 children who completed the 14-day follow-up, an adequate clinical and parasitological response was observed in 316 patients (88.5%) and early and late failures occurred in 18 (5%) and 23 (6.4%, mostly due to recrudescence) patients, respectively. {T}he majority of clinical isolates were characterized as "quadruple" (n = 196, 55.2%; {N}51{I}-{C}59{R}-{S}108{N} in {DHFR} and {A}437{G} in {DHPS}) or "triple" mutants (n = 97,27.3%; {N}51{I}-{C}59{R}-{S}108{N} in {DHFR} and wild-type {DHPS}; {S}108{N} + {N}51{I} or {C}59{R} in {DHFR} and {A}437{G} in {DHPS}). {W}ild-type, single mutation, and double mutation were observed in 29, 20, and 13 parasites, respectively. {T}he comparison of different sets of mutations and early or late failures did not reveal any molecular marker associated with treatment outcome when the follow-up period was limited to 14 days ({P} > 0.05). {I}n this study, the determination of dhfr-dhps genotypes was of limited value to predict the treatment outcome in individual patients, mostly due to few treatment failures and few wild-type haplotypes. {F}urther monitoring will be required to define the relationship between clinical response to {SP} therapy and parasite genotypes in our epidemiological setting.},
  keywords = {drug resistance ; mutations ; chloroquine ; antifolate drugs ; molecular markers ; {CAMEROUN}},
  booktitle = {},
  journal = {{A}cta {T}ropica},
  volume = {103},
  numero = {2},
  pages = {81--89},
  ISSN = {0001-706{X}},
  year = {2007},
  DOI = {10.1016/j.actatropica.2007.04.008},
  URL = {https://www.documentation.ird.fr/hor/fdi:010040791},
}