@article{fdi:010040788, title = {{S}hort report : {D}evelopment of a molecular assay to detect predation on {A}nopheles gambiae complex larval stages}, author = {{S}chielke, {E}. and {C}ostantini, {C}arlo and {C}archini, {G}. and {S}agnon, {N}. and {P}owell, {J}. and {C}accone, {A}.}, editor = {}, language = {{ENG}}, abstract = {{W}e developed a molecular assay to detect predation on {A}nopheles gambiae sensu lato (s.l.) mosquitoes. {T}his intergenic spacer ribosomal {DNA} polymerase chain reaction assay and restriction enzyme analysis uses {A}n. gambiae-specific primers to detect mosquito {DNA} in the {DNA} extracts from whole invertebrate predators, which enables identification of species ({A}n. gambiae s.s. versus {A}n. arabiensis) and molecular forms ({M} versus {S} in {A}n. gambiae s.s.). {W}e show that {A}n. gambiae s.l. {DNA} can be detected after ingestion by members of the families {L}estidae (order {O}donata) after four hours, {L}ibellulidae (order {O}donata) after six hours, and {N}otonectidae (order {H}emiptera) after 24 hours. {T}his method is an improvement over previously published methods because of ease of execution and increased time of detection after ingestion.}, keywords = {}, booktitle = {}, journal = {{A}merican {J}ournal of {T}ropical {M}edicine and {H}ygiene}, volume = {77}, numero = {3}, pages = {464--466}, ISSN = {0002-9637}, year = {2007}, URL = {https://www.documentation.ird.fr/hor/fdi:010040788}, }