@article{fdi:010040786,
  title = {{M}olecular epidemiology of malaria in {C}ameroon. {XXVI}. {T}welve-year in vitro and molecular surveillance of pyrimethamine resistance and experimental studies to modulate pyrimethamine resistance},
  author = {{T}ahar, {R}achida and {B}asco, {L}eonardo},
  editor = {},
  language = {{ENG}},
  abstract = {{I}n vitro pyrimethamine response of {P}lastnodium fulciparum isolates and dihydrofolate reductase (dhfr) gene sequences were analyzed in 2004-2005 and compared with our previous data. {M}ost isolates (n = 103, all dhfr mutants) had 50% inhibitory concentrations ({IC}(50)s) >= 119 n{M}, and six isolates had low {IC}(50)s (five wild-type or mixed dhfr, 0.04-1.37 n{M}; one triple mutant, 6.4 n{M}). {O}f 194 isolates, only 7 had the wild-type dhfr and 187 were mutants. {T}he results of the two methods were highly concordant and indicated a significant increase ({P} < 0.05) in the prevalence of mutant, pyrimethamine-resistant {P}. falciparum between 1994 and 2005. {T}he addition of probenecid or sulfinpyrazone to pyrimethamine resulted in a slight-to-moderate decrease in the level of in vitro pyrimethamine resistance without rendering the parasites susceptible to pyrimethamine. {A}nalysis of molecular markers may be useful for the long-term surveillance of antifolate-resistant malaria.},
  keywords = {{CAMEROUN}},
  booktitle = {},
  journal = {{A}merican {J}ournal of {T}ropical {M}edicine and {H}ygiene},
  volume = {77},
  numero = {2},
  pages = {221--227},
  ISSN = {0002-9637},
  year = {2007},
  URL = {https://www.documentation.ird.fr/hor/fdi:010040786},
}