Tahar Rachida auteur aut IRD Basco Leonardo auteur aut IRD text journalArticle eng text/pdf reformatted digital access The in vitro activities of fosmidomycin derivatives, chloroquine, and pyrimethamine were assessed by the radioisotopic assay in clinical isolates of Plasmodium falciparum. In a series of experiments with RPMI 1640 medium-10% fetal bovine serum, the geometric mean 50% inhibitory concentrations (IC(50)s) (n = 34) for fosmidomycin and FR900098 were 301. nM and 118 nM, respectively. In another series of experiments, the geometric mean IC(50)s (n = 33) for fosmidomycin and TH I146 were 413 nM and 249 nM, respectively. The IC(50)s were 2-3 times lower with RPMI-10% fetal bovine serum than the IC(50)s obtained with RPMI-10% human serum. FR900098 and TH II46 were 2.6 and 1.7 times more potent, respectively, than fosmidomycin. There was no correlation between chloroquine or pyrimethamine and fosmidomycin, which suggested the absence of in vitro cross-resistance. Sequence analysis showed five amino acid substitutions, but their possible relationship with the response to fosmidomycin is not clear. Fosmidomycin derivatives are promising candidates for further development. specialized CAMEROUN 052ANOPAL04 050EPID 77 2 214-220 2007 0002-9637 https://www.documentation.ird.fr/hor/fdi:010040785 0002-9637 [F B010040785] https://www.documentation.ird.fr/hor/fdi:010040785 https://horizon.documentation.ird.fr/exl-doc/pleins_textes/divers20-08/010040785.pdf IRD - Base Horizon / Pleins textes 2007-11-06 2023-07-05 fdi:010040785 fre