Dusfour Isabelle auteur aut IRD Londeau J. auteur aut IRD Harbach R. E. auteur aut IRD Vythilingham I. auteur aut IRD Baimai V. auteur aut IRD Trung H. D. auteur aut IRD Sochanta T. auteur aut IRD Bangs M. J. auteur aut IRD Manguin Sylvie auteur aut IRD text journalArticle eng text/pdf born digital access Anopheles sundaicus s.l., a major malaria vector taxon, occurs primarily along coastal areas and on islands in Southeast Asia. Our previous studies using cytochrome oxidase 1, cytochrome-b, and internal transcribed spacer 2 markers discriminated three allopatric species: An. sundaicus s.s. in northern Borneo, An. epiroticus in Southeast Asia, and An. sundaicus E on Sumatra and Java, Indonesia. Morphological comparisons of three developmental stages did not reveal unique diagnostic characters that could reliably distinguish the three species. Therefore, we developed a multiplex polymerase chain reaction (PCR) assay based on two mitochondrial DNA markers to unambiguously identify them. This PCR was tested on 374 specimens from 24 different geographical populations, expanding our knowledge of the distribution of these species. specialized Anopheles sundaicus complex morphology multiplex polymerase chain reaction mitochondrial DNA Southeast Asia 052 44 5 723-731 2007 0022-2585 https://www.documentation.ird.fr/hor/fdi:010040771 10.1603/0022-2585(2007)44[723:PCRIOT]2.0.CO;2 0022-2585 [F B010040771] https://www.documentation.ird.fr/hor/fdi:010040771 https://www.documentation.ird.fr/intranet/publi/2007/10/010040771.pdf Accès réservé (Intranet de l'IRD) IRD - Base Horizon / Pleins textes 2007-11-06 2017-08-23 fdi:010040771 fre