@article{fdi:010040771, title = {{P}olymerase chain reaction identification of three members of the {A}nopheles sundaicus ({D}iptera : culicidae) complex, malaria vectors in southeast {A}sia}, author = {{D}usfour, {I}sabelle and {L}ondeau, {J}. and {H}arbach, {R}. {E}. and {V}ythilingham, {I}. and {B}aimai, {V}. and {T}rung, {H}. {D}. and {S}ochanta, {T}. and {B}angs, {M}. {J}. and {M}anguin, {S}ylvie}, editor = {}, language = {{ENG}}, abstract = {{A}nopheles sundaicus s.l., a major malaria vector taxon, occurs primarily along coastal areas and on islands in {S}outheast {A}sia. {O}ur previous studies using cytochrome oxidase 1, cytochrome-b, and internal transcribed spacer 2 markers discriminated three allopatric species: {A}n. sundaicus s.s. in northern {B}orneo, {A}n. epiroticus in {S}outheast {A}sia, and {A}n. sundaicus {E} on {S}umatra and {J}ava, {I}ndonesia. {M}orphological comparisons of three developmental stages did not reveal unique diagnostic characters that could reliably distinguish the three species. {T}herefore, we developed a multiplex polymerase chain reaction ({PCR}) assay based on two mitochondrial {DNA} markers to unambiguously identify them. {T}his {PCR} was tested on 374 specimens from 24 different geographical populations, expanding our knowledge of the distribution of these species.}, keywords = {{A}nopheles sundaicus complex ; morphology ; multiplex polymerase chain reaction ; mitochondrial {DNA} ; {S}outheast {A}sia}, booktitle = {}, journal = {{J}ournal of {M}edical {E}ntomology}, volume = {44}, numero = {5}, pages = {723--731}, ISSN = {0022-2585}, year = {2007}, DOI = {10.1603/0022-2585(2007)44[723:{PCRIOT}]2.0.{CO};2}, URL = {https://www.documentation.ird.fr/hor/fdi:010040771}, }