Thiaba Samba Ramatoulaye auteur aut IRD Neyra Marc auteur aut IRD Lesueur D. auteur aut IRD text journalArticle eng text/pdf born digital access Polymerase Chain Reaction/Restriction Fragment Length Polymorphism (PCR/RFLP) of the InterGenic Spacer (IGS) between rDNA 16S and 23S was used to identify indigenous strains nodulating four clones of Acacia mangium-Acacia auriculiformis hybrids cultivated in non-sterilized sandy soil from Sangalkam (Senegal) under greenhouse conditions. The experiment was for 4 months. The analysis of restriction fragment length polymorphism obtained with MspI and HaeIII restriction enzymes allowed the identification of 15 different IGS Groups with a distribution which significantly differed according to the clone of the hybrid (strains of one clone can belong to three and five different IGS Groups). Three large multi-lobed nodules were obtained on the root system of clone 3.26 within 5 months. Also, the nature of the rhizobia contained in each lobe was determined. The results showed that the lobes of large nodules can be occupied by one or two strains and the nodules analysed were mainly occupied by those belonging to IGS Group 12. specialized multi lobed nodules Acacia mangium A. auriculiformis hybrid PCR/RFLP of IGS nodule occupancy 084 23 10 1485-1488 2007 0959-3993 https://www.documentation.ird.fr/hor/fdi:010040760 10.1007/s11274-007-9373-z 0959-3993 [F B010040760] https://www.documentation.ird.fr/hor/fdi:010040760 https://www.documentation.ird.fr/intranet/publi/2007/10/010040760.pdf Accès réservé (Intranet de l'IRD) IRD - Base Horizon / Pleins textes 2007-11-06 2017-08-23 fdi:010040760 fre