@article{fdi:010040760, title = {{N}atural nodulation of {A}cacia mangium-{A}cacia auriculiformis hybrids : distribution of the indigenous strains in the nodules}, author = {{T}hiaba {S}amba, {R}amatoulaye and {N}eyra, {M}arc and {L}esueur, {D}.}, editor = {}, language = {{ENG}}, abstract = {{P}olymerase {C}hain {R}eaction/{R}estriction {F}ragment {L}ength {P}olymorphism ({PCR}/{RFLP}) of the {I}nter{G}enic {S}pacer ({IGS}) between r{DNA} 16{S} and 23{S} was used to identify indigenous strains nodulating four clones of {A}cacia mangium-{A}cacia auriculiformis hybrids cultivated in non-sterilized sandy soil from {S}angalkam ({S}enegal) under greenhouse conditions. {T}he experiment was for 4 months. {T}he analysis of restriction fragment length polymorphism obtained with {M}sp{I} and {H}ae{III} restriction enzymes allowed the identification of 15 different {IGS} {G}roups with a distribution which significantly differed according to the clone of the hybrid (strains of one clone can belong to three and five different {IGS} {G}roups). {T}hree large multi-lobed nodules were obtained on the root system of clone 3.26 within 5 months. {A}lso, the nature of the rhizobia contained in each lobe was determined. {T}he results showed that the lobes of large nodules can be occupied by one or two strains and the nodules analysed were mainly occupied by those belonging to {IGS} {G}roup 12.}, keywords = {multi lobed nodules ; {A}cacia mangium ; {A}. auriculiformis ; hybrid ; {PCR}/{RFLP} of {IGS} ; nodule occupancy}, booktitle = {}, journal = {{W}orld {J}ournal of {M}icrobiology and {B}iotechnology}, volume = {23}, numero = {10}, pages = {1485--1488}, ISSN = {0959-3993}, year = {2007}, DOI = {10.1007/s11274-007-9373-z}, URL = {https://www.documentation.ird.fr/hor/fdi:010040760}, }