Lardeux Frédéric auteur aut IRD Quispe V. auteur aut IRD Tejerina Rosenka auteur aut IRD Rodriguez R. auteur aut IRD Torrez Libia auteur aut IRD Bouchité Bernard auteur aut IRD Chavez T. auteur aut IRD text journalArticle eng text/pdf born digital access Anopheles pseudopunctipennis is one of the main malaria vectors in the Andean regions of South America. Few experimental data exist on this species because it is not very available in laboratories due to its eurygamic status that makes colony maintenance difficult. Indeed, individuals do not mate in the confined space of insectary cages. To avoid this problem, forced artificial mating can be used. However, this technique is time consuming, requires a well-trained technician, and is inadequate for easy mass production, which is sometimes necessary for certain experimental works. This study presents a technique based on exposure of adult mosquitoes to a blue stroboscopic light for 20 min during several nights, which encourages them to copulate naturally under laboratory conditions. After some generations, a self-free-mating strain was obtained. The technique is simple, inexpensive and is probably effective whatever the An. pseudopunctipennis strain considered. specialized anopheles pseudopunctipennis colonization forced mating natural mating insectary 052 020 330 8 571-575 2007 1631-0691 https://www.documentation.ird.fr/hor/fdi:010040728 10.1016/j.crvi.2007.04.002 1631-0691 [F B010040728] https://www.documentation.ird.fr/hor/fdi:010040728 https://www.documentation.ird.fr/intranet/publi/2007/10/010040728.pdf Accès réservé (Intranet de l'IRD) IRD - Base Horizon / Pleins textes 2007-11-06 2017-08-23 fdi:010040728 fre